المؤلفون: Ping Xiao, Hongzhi Huang, Xiang Li, Jianwei Chen, Jin-ao Duan

المصدر: BMC Complementary and Alternative Medicine, Vol 19, Iss 1, Pp 1-9 (2019)

مصطلحات موضوعية: Radix isatidis protein, Oxidative damage, Antioxidant activity, Protein composition, D-galactose, Other systems of medicine, RZ201-999

الوصف: Abstract Background Radix isatidis (Isatis indigotica Fort.) is an ancient medicinal herb, which has been applied to the prevention and treatment of influenza virus since ancient times. In recent years, the antioxidant activity of Radix isatidis has been widely concerned by researchers. Our previous studies have shown that Radix isatidis protein (RIP) has good antioxidant activity in vitro. In this study, the composition of the protein was characterized and its antioxidant activity in vivo was evaluated. Methods The model of oxidative damage in mice was established by subcutaneous injection of D-galactose for 7 weeks. Commercially available kits were used to determine the content of protein and several oxidation indexes in different tissues of mice. The tissue samples were stained with hematoxylin and eosin (H&E) and the pathological changes were observed by optical microscope. The molecular weight of RIP was analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The amino acid composition of RIP was determined by a non-derivative method developed by our research group. Results RIP significantly increased the activities of antioxidant enzymes such as SOD, CAT, GSH-Px and total antioxidant capability (TAOC) but decreased the MDA level in the serum, kidney and liver. H&E stained sections of liver and kidney revealed D-galactose could cause serious injury and RIP could substantially attenuate the injury. The analysis of SDS-PAGE showed that four bands with molecular weights of 19.2 kDa, 21.5 kDa, 24.8 kDa and 40.0 kDa were the main protein components of RIP. Conclusions The results suggested that RIP had excellent antioxidant activity, which could be explored as a health-care product to retard aging and a good source of protein nutrition for human consumption.

وصف الملف: electronic resource

العلاقة: http://link.springer.com/article/10.1186/s12906-019-2726-yTest; https://doaj.org/toc/1472-6882Test

الوصول الحر: https://doaj.org/article/a62bb3395628432c980a95e057d7fc0aTest

المؤلفون: Yizhong Yan, Jianjun Li, Yiya Zhang, Xiaozhen Peng, Tianyao Guo, Jirong Wang, Weijun Hu, Zhigui Duan, Xianchun Wang

المصدر: Biological Research, Vol 47, Iss 0, Pp 1-11 (2014)

مصطلحات موضوعية: Black widow spider (L. tredecimguttatus), Protein composition, Egg, Molecular mechanism, Property analysis, Toxicity, Biology (General), QH301-705.5

الوصف: BACKGROUND: Black widow spider (L. tredecimguttatus) has toxic components not only in the venomous glands, but also in other parts of the body and its eggs. It is biologically important to investigate the molecular basis of the egg toxicity. RESULTS: In the present work, an aqueous extract was prepared from the eggs of the spider and characterized using multiple physiological and biochemical strategies. Gel electrophoresis and mass spectrometry demonstrated that the eggs are rich in high-molecular-mass proteins and the peptides below 5 kDa. The lyophilized extract of the eggs had a protein content of 34.22% and was shown to have a strong toxicity towards mammals and insects. When applied at a concentration of 0.25 mg/mL, the extract could completely block the neuromuscular transmission in mouse isolated phrenic nerve-hemidiaphragm preparations within 12.0 ± 1.5 min. Using whole-cell patch-clamp technique, the egg extract was demonstrated to be able to inhibit the voltage-activated Na+, K+and Ca2+ currents in rat DRG neurons. In addition, the extract displayed activities of multiple hydrolases. Finally, the molecular basis of the egg toxicity was discussed. CONCLUSIONS: The eggs of black widow spiders are rich in proteinous compounds particularly the high-molecular-mass proteins with different types of biological activity The neurotoxic and other active compounds in the eggs are believed to play important roles in the eggs' toxic actions.

وصف الملف: electronic resource

العلاقة: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100017&lng=en&tlng=enTest; https://doaj.org/toc/0716-9760Test

الوصول الحر: https://doaj.org/article/914e73c384f9432c912389437bca67d6Test

المؤلفون: Yiya Zhang, Tianyao Guo, Zhigui Duan, Jirong Wang, Weijun Hu, Xiaozhen Peng, Yizhong Yan, Xianchun Wang, Jianjun Li

المصدر: Biological Research, Vol 47, Iss 0, Pp 1-11 (2014)
Biological Research
Biological Research, Volume: 47, Pages: 1-11, Published: 2014
Biological Research v.47 2014
SciELO Chile
CONICYT Chile
instacron:CONICYT

مصطلحات موضوعية: Black widow spider (L. tredecimguttatus), Neuromuscular transmission, Cockroaches, Voltage-Gated Sodium Channels, Arthropod Proteins, Molecular mechanism, Toxicology, Mice, Animal Shells, biology.animal, Ganglia, Spinal, Animals, Black Widow Spider, Egg, Protein composition, lcsh:QH301-705.5, Ovum, Medicine(all), Gel electrophoresis, Spider, Cockroach, Agricultural and Biological Sciences(all), biology, Toxicity, Biochemistry, Genetics and Molecular Biology(all), Tissue Extracts, Biological activity, Rats, Phrenic Nerve, Biochemistry, lcsh:Biology (General), Potassium Channels, Voltage-Gated, embryonic structures, Property analysis, Calcium Channels, Developmental biology, Research Article

الوصف: BACKGROUND: Black widow spider (L. tredecimguttatus) has toxic components not only in the venomous glands, but also in other parts of the body and its eggs. It is biologically important to investigate the molecular basis of the egg toxicity. RESULTS: In the present work, an aqueous extract was prepared from the eggs of the spider and characterized using multiple physiological and biochemical strategies. Gel electrophoresis and mass spectrometry demonstrated that the eggs are rich in high-molecular-mass proteins and the peptides below 5 kDa. The lyophilized extract of the eggs had a protein content of 34.22% and was shown to have a strong toxicity towards mammals and insects. When applied at a concentration of 0.25 mg/mL, the extract could completely block the neuromuscular transmission in mouse isolated phrenic nerve-hemidiaphragm preparations within 12.0 ± 1.5 min. Using whole-cell patch-clamp technique, the egg extract was demonstrated to be able to inhibit the voltage-activated Na+, K+and Ca2+ currents in rat DRG neurons. In addition, the extract displayed activities of multiple hydrolases. Finally, the molecular basis of the egg toxicity was discussed. CONCLUSIONS: The eggs of black widow spiders are rich in proteinous compounds particularly the high-molecular-mass proteins with different types of biological activity The neurotoxic and other active compounds in the eggs are believed to play important roles in the eggs' toxic actions.

وصف الملف: text/html

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9e4d75181f16584fedfc7d10f80b3c0eTest
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100017&lng=en&tlng=enTest

المؤلفون: Jian Wen Hu, Le Feng, Hao Wang, Fang Wang, Chun Lei Xie

المصدر: Proteome Science, Vol 10, Iss 1, p 15 (2012)
Proteome Science

مصطلحات موضوعية: medicine.medical_specialty, endocrine system diseases, genetic structures, Protein profile, Biochemistry, Diabetes mellitus, Ophthalmology, medicine, Quantitative proteomics, Proliferative diabetic retinopathy, cardiovascular diseases, Vitreoretinal diseases, lcsh:QH573-671, Molecular Biology, Vitreous humour, business.industry, lcsh:Cytology, Research, Disease mechanisms, Corneal Transplant, Diabetic retinopathy, Protein composition, medicine.disease, eye diseases, Proteome, Marker proteins, sense organs, business

الوصف: Background Diabetes can lead to serious microvascular complications such as proliferative diabetic retinopathy (PDR), which results in severe vision loss. The diabetes-induced alterations in the vitreous protein composition in diabetic patients with PDR may be responsible for the presence of PDR. The vitreous humour can be utilised in a variety of studies aimed toward the discovery of new targets for the treatment or prevention of PDR and the identification of novel disease mechanisms. The aim of this study was to compare the protein profile of vitreous humour from diabetic patients with PDR with that of vitreous humour from normal human eyes donated for corneal transplant. Results Vitreous humour from type 2 diabetic patients with PDR (n = 10) and from normal human eyes donated for corneal transplant (n = 10) were studied. The comparative proteomic analysis was performed using two-dimensional fluorescence difference gel electrophoresis (2-D DIGE). Differentially produced proteins (abundance ratio > 2 or < -2, p < 0.01) were identified by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry (MALDI-TOF MS) and MALDI-TOF tandem mass spectrometry. A total of 1242 protein spots were detected on the 2-D master gel of the samples, and 57 spots that exhibited statistically significant variations were successfully identified. The spots corresponded to peptide fragments of 29 proteins, including 8 proteins that increased and 21 proteins that decreased in PDR. Excluding the serum proteins from minor vitreous haemorrhage, 19 proteins were found to be differentially produced in PDR patients compared with normal subjects; 6 of these proteins have never been reported to be differentially expressed in PDR vitreous: N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 (DDAH 1), tubulin alpha-1B chain, gamma-enolase, cytosolic acyl coenzyme A thioester hydrolase, malate dehydrogenase and phosphatidylethanolamine-binding protein 1 (PEBP 1). The differential production of pigment epithelium-derived factor (PEDF) and clusterin was confirmed by Western blot analysis. Conclusions These data provide an in-depth analysis of the human vitreous proteome and reveal protein alterations that are possibly involved in the pathogenesis of PDR. Further investigation of these special proteins may provide potential new targets for the treatment or the prevention of PDR.

الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_dedup___::693572c5989a261a05e4d1f69a03493dTest
http://www.proteomesci.com/content/10/1/15Test