Identification of novel vascular markers through gene expression profiling of tumor-derived endothelium
| العنوان: | Identification of novel vascular markers through gene expression profiling of tumor-derived endothelium |
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| المؤلفون: | Carmen Ghilardi, Giovanna Chiorino, Romina Dossi, Maria Rosa Bani, Zsuzsanna Nagy, Raffaella Giavazzi |
| المصدر: | BMC Genomics, Vol 9, Iss 1, p 201 (2008) BMC Genomics |
| بيانات النشر: | BMC, 2008. |
| سنة النشر: | 2008 |
| مصطلحات موضوعية: | Male, Endothelium, lcsh:QH426-470, lcsh:Biotechnology, In situ hybridization, Biology, Transcriptome, chemistry.chemical_compound, Antigens, Neoplasm, Cell Line, Tumor, Neoplasms, lcsh:TP248.13-248.65, Adrenal Glands, Endopeptidases, Biomarkers, Tumor, Genetics, medicine, Humans, Trypsin, Oligonucleotide Array Sequence Analysis, Ovarian Neoplasms, Membrane Glycoproteins, GPNMB, Microarray analysis techniques, Gene Expression Profiling, Serine Endopeptidases, Endothelial Cells, Membrane Proteins, Tumor-Derived, Cell biology, Gene Expression Regulation, Neoplastic, Gene expression profiling, Vascular endothelial growth factor, ADAM Proteins, lcsh:Genetics, medicine.anatomical_structure, chemistry, Gelatinases, Female, Research Article, Biotechnology |
| الوصف: | BackgroundTargeting tumor angiogenesis and vasculature is a promising strategy for the inhibition of tumor growth and dissemination. Evidence suggests that tumor vasculature expresses unique markers that distinguish it from normal vasculature. Our efforts focused on the molecular characterization of endothelial cells (EC) in the search for selective markers of tumor vasculature that might be helpful for the development of effective therapeutic approaches.ResultsWe investigated by microarray analysis the gene expression profiles of EC purified and cultured from tumor (ovarian carcinoma [HOC-EC]) and normal (human adrenal gland [HA-EC]) tissue specimens. We found distinct transcriptional features characterizing the EC of different origin, and identified 158 transcripts highly expressed by HOC-EC. We analyzed four of these genes, ADAM23, FAP, GPNMB and PRSS3, which were not previously known to be expressed by endothelium.In vitroexperiments confirmed the higher expression of the selected genes in tumor-derived endothelium with no expression in tumor cells.In vivoinvestigation byin situhybridization established that ADAM23, GPNMB and PRSS3 expression is localized on blood vessels of human cancer specimens.ConclusionThese findings elucidate some of the molecular features of the tumor endothelium. Comparative transcriptomic analysis allowed us to determine molecular differences of tumor and normal tissue-derived endothelium and to identify novel markers that might be exploited to selectively target tumor vasculature. |
| اللغة: | English |
| تدمد: | 1471-2164 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3ef3e9c249f47bed7c5e40bd32c0dda1Test http://www.biomedcentral.com/1471-2164/9/201Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3ef3e9c249f47bed7c5e40bd32c0dda1 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14712164 |
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