دورية أكاديمية
Robust generation of human-chambered cardiac organoids from pluripotent stem cells for improved modelling of cardiovascular diseases
| العنوان: | Robust generation of human-chambered cardiac organoids from pluripotent stem cells for improved modelling of cardiovascular diseases |
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| المؤلفون: | Beatrice Xuan Ho, Jeremy Kah Sheng Pang, Ying Chen, Yuin-Han Loh, Omer An, Henry He Yang, Veerabrahma Pratap Seshachalam, Judice L. Y. Koh, Woon-Khiong Chan, Shi Yan Ng, Boon Seng Soh |
| المصدر: | Stem Cell Research & Therapy, Vol 13, Iss 1, Pp 1-18 (2022) |
| بيانات النشر: | BMC, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Medicine (General) LCC:Biochemistry |
| مصطلحات موضوعية: | Cardiac progenitor, Chambered cardiac organoid, Disease modelling, Human pluripotent stem cells, Single-cell RNA sequencing, Medicine (General), R5-920, Biochemistry, QD415-436 |
| الوصف: | Abstract Background Tissue organoids generated from human pluripotent stem cells are valuable tools for disease modelling and to understand developmental processes. While recent progress in human cardiac organoids revealed the ability of these stem cell-derived organoids to self-organize and intrinsically formed chamber-like structure containing a central cavity, it remained unclear the processes involved that enabled such chamber formation. Methods Chambered cardiac organoids (CCOs) differentiated from human embryonic stem cells (H7) were generated by modulation of Wnt/ß-catenin signalling under fully defined conditions, and several growth factors essential for cardiac progenitor expansion. Transcriptomic profiling of day 8, day 14 and day 21 CCOs was performed by quantitative PCR and single-cell RNA sequencing. Endothelin-1 (EDN1) known to induce oxidative stress in cardiomyocytes was used to induce cardiac hypertrophy in CCOs in vitro. Functional characterization of cardiomyocyte contractile machinery was performed by immunofluorescence staining and analysis of brightfield and fluorescent video recordings. Quantitative PCR values between groups were compared using two-tailed Student’s t tests. Cardiac organoid parameters comparison between groups was performed using two-tailed Mann–Whitney U test when sample size is small; otherwise, Welch’s t test was used. Comparison of calcium kinetics parameters derived from the fluorescent data was performed using two-tailed Student’s t tests. Results Importantly, we demonstrated that a threshold number of cardiac progenitor was essential to line the circumference of the inner cavity to ensure proper formation of a chamber within the organoid. Single-cell RNA sequencing revealed improved maturation over a time course, as evidenced from increased mRNA expression of cardiomyocyte maturation genes, ion channel genes and a metabolic shift from glycolysis to fatty acid ß-oxidation. Functionally, CCOs recapitulated clinical cardiac hypertrophy by exhibiting thickened chamber walls, reduced fractional shortening, and increased myofibrillar disarray upon treatment with EDN1. Furthermore, electrophysiological assessment of calcium transients displayed tachyarrhythmic phenotype observed as a consequence of rapid depolarization occurring prior to a complete repolarization. Conclusions Our findings shed novel insights into the role of progenitors in CCO formation and pave the way for the robust generation of cardiac organoids, as a platform for future applications in disease modelling and drug screening in vitro. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1757-6512 |
| العلاقة: | https://doaj.org/toc/1757-6512Test |
| DOI: | 10.1186/s13287-022-03215-1 |
| الوصول الحر: | https://doaj.org/article/05148704b297449b9cdbc013e856a7a4Test |
| رقم الانضمام: | edsdoj.05148704b297449b9cdbc013e856a7a4 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 17576512 |
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| DOI: | 10.1186/s13287-022-03215-1 |