دورية أكاديمية
Electrospun micro- and nanofiber tubes for functional nervous regeneration in sciatic nerve transections
| العنوان: | Electrospun micro- and nanofiber tubes for functional nervous regeneration in sciatic nerve transections |
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| المؤلفون: | Amadio Stefano, Taraballi Francesca, Del Carro Ubaldo, Lowery Joseph, Cunha Carla, Panseri Silvia, Vescovi Angelo, Gelain Fabrizio |
| المصدر: | BMC Biotechnology, Vol 8, Iss 1, p 39 (2008) |
| بيانات النشر: | BMC |
| سنة النشر: | 2008 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Biotechnology, TP248.13-248.65 |
| الوصف: | Background Although many nerve prostheses have been proposed in recent years, in the case of consistent loss of nervous tissue peripheral nerve injury is still a traumatic pathology that may impair patient's movements by interrupting his motor-sensory pathways. In the last few decades tissue engineering has opened the door to new approaches;: however most of them make use of rigid channel guides that may cause cell loss due to the lack of physiological local stresses exerted over the nervous tissue during patient's movement. Electrospinning technique makes it possible to spin microfiber and nanofiber flexible tubular scaffolds composed of a number of natural and synthetic components, showing high porosity and remarkable surface/volume ratio. Results In this study we used electrospun tubes made of biodegradable polymers (a blend of PLGA/PCL) to regenerate a 10-mm nerve gap in a rat sciatic nerve in vivo . Experimental groups comprise lesioned animals (control group) and lesioned animals subjected to guide conduits implantated at the severed nerve stumps, where the tubular scaffolds are filled with saline solution. Four months after surgery, sciatic nerves failed to reconnect the two stumps of transected nerves in the control animal group. In most of the treated animals the electrospun tubes induced nervous regeneration and functional reconnection of the two severed sciatic nerve tracts. Myelination and collagen IV deposition have been detected in concurrence with regenerated fibers. No significant inflammatory response has been found. Neural tracers revealed the re-establishment of functional neuronal connections and evoked potential results showed the reinnervation of the target muscles in the majority of the treated animals. Conclusion Corroborating previous works, this study indicates that electrospun tubes, with no additional biological coating or drug loading treatment, are promising scaffolds for functional nervous regeneration. They can be knitted in meshes and various frames depending on the ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1472-6750 |
| العلاقة: | http://www.biomedcentral.com/1472-6750/8/39Test; https://doaj.org/toc/1472-6750Test; https://doaj.org/article/0cea4a942c324a06b435fb179beb03b7Test |
| DOI: | 10.1186/1472-6750-8-39 |
| الإتاحة: | https://doi.org/10.1186/1472-6750-8-39Test https://doaj.org/article/0cea4a942c324a06b435fb179beb03b7Test |
| رقم الانضمام: | edsbas.54A76393 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 14726750 |
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| DOI: | 10.1186/1472-6750-8-39 |