دورية أكاديمية
Seladin-1 expression is regulated by promoter methylation in adrenal cancer
| العنوان: | Seladin-1 expression is regulated by promoter methylation in adrenal cancer |
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| المؤلفون: | Mannelli Massimo, Arvia Rosaria, Deledda Cristiana, Gelmini Stefania, Luciani Paola, Malentacchi Francesca, Simi Lisa, Peri Alessandro, Orlando Claudio |
| المصدر: | BMC Cancer, Vol 10, Iss 1, p 201 (2010) |
| بيانات النشر: | BMC |
| سنة النشر: | 2010 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Neoplasms. Tumors. Oncology. Including cancer and carcinogens, RC254-282 |
| الوصف: | Background Seladin-1 overexpression exerts a protective mechanism against apoptosis. Seladin-1 mRNA is variably expressed in normal human tissues. Adrenal glands show the highest levels of seladin-1 expression, which are significantly reduced in adrenal carcinomas (ACC). Since up to now seladin-1 mutations were not described, we investigated whether promoter methylation could account for the down-regulation of seladin-1 expression in ACC. Methods A methylation sensitive site was identified in the seladin-1 gene. We treated DNA extracted from two ACC cell lines (H295R and SW13) with the demethylating agent 5-Aza-2-deoxycytidine (5-Aza). Furthermore, to evaluate the presence of an epigenetic regulation also 'in vivo', seladin-1 methylation and its mRNA expression were measured in 9 ACC and in 5 normal adrenal glands. Results The treatment of cell lines with 5-Aza induced a significant increase of seladin-1 mRNA expression in H295R (fold increase, F.I. = 1.8; p = 0.02) and SW13 (F.I. = 2.9; p = 0.03). In ACC, methylation density of seladin-1 promoter was higher (2682 ± 686) than in normal adrenal glands (362 ± 97; p = 0.02). Seladin-1 mRNA expression in ACC (1452 ± 196) was significantly lower than in normal adrenal glands (3614 ± 949; p = 0.01). Conclusion On this basis, methylation could be involved in the altered pattern of seladin-1 gene expression in ACC. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1471-2407 |
| العلاقة: | http://www.biomedcentral.com/1471-2407/10/201Test; https://doaj.org/toc/1471-2407Test; https://doaj.org/article/b3a9cf1c078043c6a2abd6ad777a08a5Test |
| DOI: | 10.1186/1471-2407-10-201 |
| الإتاحة: | https://doi.org/10.1186/1471-2407-10-201Test https://doaj.org/article/b3a9cf1c078043c6a2abd6ad777a08a5Test |
| رقم الانضمام: | edsbas.BB19AD40 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 14712407 |
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| DOI: | 10.1186/1471-2407-10-201 |