دورية أكاديمية
Immune-instructive copolymer scaffolds using plant-derived nanoparticles to promote bone regeneration
| العنوان: | Immune-instructive copolymer scaffolds using plant-derived nanoparticles to promote bone regeneration |
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| المؤلفون: | Suliman, Salwa, Mieszkowska, Anna, Folkert, Justyna, Rana, Neha, Mohamed-Ahmed, Samih Salah Eldin Mahgoub, Fuoco, Tiziana, Finne-Wistrand, Anna, Dirscherl, Kai, Jørgensen, Bodil, Mustafa, Kamal Babikeir Elnour, Gurzawska-Comis, Katarzyna |
| المصدر: | 12 ; Inflammation and Regeneration ; 42 |
| بيانات النشر: | BMC |
| سنة النشر: | 2022 |
| المجموعة: | University of Bergen: Bergen Open Research Archive (BORA-UiB) |
| الوصف: | Background Age-driven immune signals cause a state of chronic low-grade inflammation and in consequence affect bone healing and cause challenges for clinicians when repairing critical-sized bone defects in elderly patients. Methods Poly(L-lactide-co-ɛ-caprolactone) (PLCA) scaffolds are functionalized with plant-derived nanoparticles from potato, rhamnogalacturonan-I (RG-I), to investigate their ability to modulate inflammation in vitro in neutrophils and macrophages at gene and protein levels. The scaffolds’ early and late host response at gene, protein and histological levels is tested in vivo in a subcutaneous rat model and their potential to promote bone regeneration in an aged rodent was tested in a critical-sized calvaria bone defect. Significant differences were tested using one-way ANOVA, followed by a multiple-comparison Tukey’s test with a p value ≤ 0.05 considered significant. Results Gene expressions revealed PLCA scaffold functionalized with plant-derived RG-I with a relatively higher amount of galactose than arabinose (potato dearabinated (PA)) to reduce the inflammatory state stimulated by bacterial LPS in neutrophils and macrophages in vitro. LPS-stimulated neutrophils show a significantly decreased intracellular accumulation of galectin-3 in the presence of PA functionalization compared to Control (unmodified PLCA scaffolds). The in vivo gene and protein expressions revealed comparable results to in vitro. The host response is modulated towards anti-inflammatory/ healing at early and late time points at gene and protein levels. A reduced foreign body reaction and fibrous capsule formation is observed when PLCA scaffolds functionalized with PA were implanted in vivo subcutaneously. PLCA scaffolds functionalized with PA modulated the cytokine and chemokine expressions in vivo during early and late inflammatory phases. PLCA scaffolds functionalized with PA implanted in calvaria defects of aged rats downregulating pro-inflammatory gene markers while promoting osteogenic markers after 2 weeks in vivo. ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1880-9693 |
| العلاقة: | urn:issn:1880-9693; https://hdl.handle.net/11250/3038669Test; https://doi.org/10.1186/s41232-022-00196-9Test; cristin:2026059; Inflammation and Regeneration. 2022, 42, 12. |
| DOI: | 10.1186/s41232-022-00196-9 |
| الإتاحة: | https://doi.org/10.1186/s41232-022-00196-9Test https://hdl.handle.net/11250/3038669Test |
| حقوق: | Navngivelse 4.0 Internasjonal ; http://creativecommons.org/licenses/by/4.0/deed.noTest ; Copyright 2022 The Author(s) |
| رقم الانضمام: | edsbas.630F067D |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 18809693 |
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| DOI: | 10.1186/s41232-022-00196-9 |