دورية أكاديمية
Genomic and transcriptomic insights into the thermo-regulated biosynthesis of validamycin in Streptomyces hygroscopicus 5008
| العنوان: | Genomic and transcriptomic insights into the thermo-regulated biosynthesis of validamycin in Streptomyces hygroscopicus 5008 |
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| المؤلفون: | Wu Hang, Qu Shuang, Lu Chenyang, Zheng Huajun, Zhou Xiufen, Bai Linquan, Deng Zixin |
| المصدر: | BMC Genomics, Vol 13, Iss 1, p 337 (2012) |
| بيانات النشر: | BMC |
| سنة النشر: | 2012 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Validamycin, Genome, Transcriptome, Streptomyces, Metabolism, Thermo-regulation, Biotechnology, TP248.13-248.65, Genetics, QH426-470 |
| الوصف: | Background Streptomyces hygroscopicus 5008 has been used for the production of the antifungal validamycin/jinggangmycin for more than 40 years. A high yield of validamycin is achieved by culturing the strain at 37°C, rather than at 30°C for normal growth and sporulation. The mechanism(s) of its thermo-regulated biosynthesis was largely unknown. Results The 10,383,684-bp genome of strain 5008 was completely sequenced and composed of a linear chromosome, a 164.57-kb linear plasmid, and a 73.28-kb circular plasmid. Compared with other Streptomyces genomes, the chromosome of strain 5008 has a smaller core region and shorter terminal inverted repeats, encodes more α/β hydrolases, major facilitator superfamily transporters, and Mg 2+ /Mn 2+ -dependent regulatory phosphatases. Transcriptomic analysis revealed that the expression of 7.5% of coding sequences was increased at 37°C, including biosynthetic genes for validamycin and other three secondary metabolites. At 37°C, a glutamate dehydrogenase was transcriptionally up-regulated, and further proved its involvement in validamycin production by gene replacement. Moreover, efficient synthesis and utilization of intracellular glutamate were noticed in strain 5008 at 37°C, revealing glutamate as the nitrogen source for validamycin biosynthesis. Furthermore, a SARP-family regulatory gene with enhanced transcription at 37°C was identified and confirmed to be positively involved in the thermo-regulation of validamycin production by gene inactivation and transcriptional analysis. Conclusions Strain 5008 seemed to have evolved with specific genomic components to facilitate the thermo-regulated validamycin biosynthesis. The data obtained here will facilitate future studies for validamycin yield improvement and industrial bioprocess optimization. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1471-2164 |
| العلاقة: | http://www.biomedcentral.com/1471-2164/13/337Test; https://doaj.org/toc/1471-2164Test; https://doaj.org/article/bbf936bf8d3a46baa1fad2dae3a2ffabTest |
| DOI: | 10.1186/1471-2164-13-337 |
| الإتاحة: | https://doi.org/10.1186/1471-2164-13-337Test https://doaj.org/article/bbf936bf8d3a46baa1fad2dae3a2ffabTest |
| رقم الانضمام: | edsbas.FC33FA6C |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 14712164 |
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| DOI: | 10.1186/1471-2164-13-337 |