دورية أكاديمية
Development of cassava common mosaic virus-based vector for protein expression and gene editing in cassava
| العنوان: | Development of cassava common mosaic virus-based vector for protein expression and gene editing in cassava |
|---|---|
| المؤلفون: | Decai Tuo, Yuan Yao, Pu Yan, Xin Chen, Feihong Qu, Weiqian Xue, Jinping Liu, Hua Kong, Jianchun Guo, Hongguang Cui, Zhaoji Dai, Wentao Shen |
| المصدر: | Plant Methods, Vol 19, Iss 1, Pp 1-12 (2023) |
| بيانات النشر: | BMC, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Plant culture LCC:Biology (General) |
| مصطلحات موضوعية: | Cassava, Cassava common mosaic virus, Protein overexpression, Genome editing, Plant culture, SB1-1110, Biology (General), QH301-705.5 |
| الوصف: | Abstract Background Plant virus vectors designed for virus-mediated protein overexpression (VOX), virus-induced gene silencing (VIGS), and genome editing (VIGE) provide rapid and cost-effective tools for functional genomics studies, biotechnology applications and genome modification in plants. We previously reported that a cassava common mosaic virus (CsCMV, genus Potexvirus)-based VIGS vector was used for rapid gene function analysis in cassava. However, there are no VOX and VIGE vectors available in cassava. Results In this study, we developed an efficient VOX vector (CsCMV2-NC) for cassava by modifying the CsCMV-based VIGS vector. Specifically, the length of the duplicated putative subgenomic promoter (SGP1) of the CsCMV CP gene was increased to improve heterologous protein expression in cassava plants. The modified CsCMV2-NC-based VOX vector was engineered to express genes encoding green fluorescent protein (GFP), bacterial phytoene synthase (crtB), and Xanthomonas axonopodis pv. manihotis (Xam) type III effector XopAO1 for viral infection tracking, carotenoid biofortification and Xam virulence effector identification in cassava. In addition, we used CsCMV2-NC to deliver single guide RNAs (gMePDS1/2) targeting two loci of the cassava phytoene desaturase gene (MePDS) in Cas9-overexpressing transgenic cassava lines. The CsCMV-gMePDS1/2 efficiently induced deletion mutations of the targeted MePDS with the albino phenotypes in systemically infected cassava leaves. Conclusions Our results provide a useful tool for rapid and efficient heterologous protein expression and guide RNA delivery in cassava. This expands the potential applications of CsCMV-based vector in gene function studies, biotechnology research, and precision breeding for cassava. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1746-4811 |
| العلاقة: | https://doaj.org/toc/1746-4811Test |
| DOI: | 10.1186/s13007-023-01055-5 |
| الوصول الحر: | https://doaj.org/article/f5010f00262144018a315b9b093ceb83Test |
| رقم الانضمام: | edsdoj.f5010f00262144018a315b9b093ceb83 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 17464811 |
|---|---|
| DOI: | 10.1186/s13007-023-01055-5 |