دورية أكاديمية
Detection of viral RNA fragments in human iPSC cardiomyocytes following treatment with extracellular vesicles from SARS-CoV-2 coding sequence overexpressing lung epithelial cells
| العنوان: | Detection of viral RNA fragments in human iPSC cardiomyocytes following treatment with extracellular vesicles from SARS-CoV-2 coding sequence overexpressing lung epithelial cells |
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| المؤلفون: | Kwon, Youjeong, Nukala, Sarath Babu, Srivastava, Shubhi, Miyamoto, Hiroe, Ismail, Nur Izzah, Jousma, Jordan, Rehman, Jalees, Ong, Sang-Bing, Lee, Won Hee, Ong, Sang-Ging |
| المساهمون: | Univ Arizona, Dept Basic Med Sci, Coll Med Phoenix |
| المصدر: | Stem cell research & therapy ; 11 ; 1 ; 514 ; United States ; International ; England |
| بيانات النشر: | BMC |
| سنة النشر: | 2020 |
| المجموعة: | The University of Arizona: UA Campus Repository |
| مصطلحات موضوعية: | COVID-19, Extracellular vesicles, iPSCs, Stem cells, Cardiomyocytes |
| الوصف: | Coronavirus disease 2019 (COVID-19) caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a global pandemic. The prevalence/severity of COVID-19 is higher among patients with cardiovascular risk factors. Despite the expression of angiotensin-converting enzyme 2 (ACE2), a receptor for SARS-CoV-2 infection, in cardiomyocytes, there has been no conclusive evidence of direct viral infection although the presence of viral genome within COVID-19 patients' hearts has been reported. Here, we overexpressed SARS-CoV-2 genes in A549 lung epithelial cells. We then isolated extracellular vesicles (EVs) and detected the presence of viral RNA within these EVs. We observed that human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) are receptive to these EVs, and viral genes were detectable in the cardiomyocytes. Accordingly, the uptake of viral RNA-harboring EVs led to an upregulation of inflammation-related genes in hiPSC-CMs. Thus, our findings indicate that SARS-CoV-2 RNA containing EVs represents an indirect route of viral RNA entry into cardiomyocytes. ; Open access article ; This item from the UA Faculty Publications collection is made available by the University of Arizona with support from the University of Arizona Libraries. If you have questions, please contact us at repository@u.library.arizona.edu. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1757-6512 |
| العلاقة: | Kwon, Y., Nukala, S. B., Srivastava, S., Miyamoto, H., Ismail, N. I., Jousma, J., . & Ong, S. G. (2020). Detection of viral RNA fragments in human iPSC cardiomyocytes following treatment with extracellular vesicles from SARS-CoV-2 coding sequence overexpressing lung epithelial cells. Stem Cell Research & Therapy, 11(1), 1-5.; http://hdl.handle.net/10150/658179Test; STEM CELL RESEARCH & THERAPY |
| DOI: | 10.1186/s13287-020-02033-7 |
| الإتاحة: | https://doi.org/10.1186/s13287-020-02033-7Test http://hdl.handle.net/10150/658179Test |
| حقوق: | © The Author(s). 2020 Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0Test/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0Test/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. ; https://creativecommons.org/licenses/by/4.0Test/ |
| رقم الانضمام: | edsbas.911E2D5 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 17576512 |
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| DOI: | 10.1186/s13287-020-02033-7 |