دورية أكاديمية
Microvessel changes after post-ischemic benign and malignant hyperemia: experimental study in rats
| العنوان: | Microvessel changes after post-ischemic benign and malignant hyperemia: experimental study in rats |
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| المؤلفون: | You Xiaofang, Li Yongdong, Cheng Yingsheng, Li Minghua, Zhao Jungong, Lu Haitao, Zhao Yuwu |
| المصدر: | BMC Neurology, Vol 10, Iss 1, p 24 (2010) |
| بيانات النشر: | BMC |
| سنة النشر: | 2010 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Neurology. Diseases of the nervous system, RC346-429 |
| الوصف: | Background The present investigation was designed to elucidate the use of dynamic contrast enhanced perfusion MR imaging (DCE pMRI) in characterizing hyperemia, including microvessel changes, and to examine whether DCE pMRI can predict benign or malignant hyperemia. Methods Sprague-Dawley rats underwent middle cerebral artery occlusion (MCAO) by intraluminal suture placement. All rats were randomized to 4 groups: MCAO for 0.5 hours followed by saline treatment (10 ml/kg; group 1); MCAO for 3 hours followed by treatment with saline (group 2) or urokinase (25000 IU/kg; group 3); and MCAO for 6 hours followed by urokinase treatment (group 4). Relative cerebral blood volume (rCBV) and relative maximum slope of increase of the signal intensity time curve (rMSI) were quantitatively analyzed from MRI. Microvessel diameter and blood-brain barrier disruption obtained by laser scanning confocal microscopy (LSCM) as well as transmission electron microscopy (TEM) were obtained for correlative study. Results Benign hyperemia was noticed only in group 1; malignant hyperemia was seen in group 3. Although the rCBV of malignant hyperemia was slightly higher than in benign hyperemia ( P > 0.05), the rMSI, on the other hand, was significantly lower ( P < 0.05). Fluoro-isothiocyanate dextran (FITC-dextran) extravasations, marked glial end-foot process swelling, and significant vasodilatation were seen in malignant hyperemia, while no or mild leakage of FITC-dextran and slight glial end-foot process swelling occurred in benign hyperemia. Conclusion Our findings indicate that DCE pMRI can characterize post-ischemic hyperemia and correlates well with microvascular damage. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1471-2377 |
| العلاقة: | http://www.biomedcentral.com/1471-2377/10/24Test; https://doaj.org/toc/1471-2377Test; https://doaj.org/article/5dd71d2f11164b3db9cbdf258e938447Test |
| DOI: | 10.1186/1471-2377-10-24 |
| الإتاحة: | https://doi.org/10.1186/1471-2377-10-24Test https://doaj.org/article/5dd71d2f11164b3db9cbdf258e938447Test |
| رقم الانضمام: | edsbas.4A2078F8 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 14712377 |
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| DOI: | 10.1186/1471-2377-10-24 |