دورية أكاديمية
Microbiological and 16S rRNA analysis of sulphite-reducing clostridia from river sediments in central Italy
| العنوان: | Microbiological and 16S rRNA analysis of sulphite-reducing clostridia from river sediments in central Italy |
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| المؤلفون: | Marcheggiani Stefania, Iaconelli Marcello, D'angelo Annamaria, Pierdominici Elio, La Rosa Giuseppina, Muscillo Michele, Equestre Michele, Mancini Laura |
| المصدر: | BMC Microbiology, Vol 8, Iss 1, p 171 (2008) |
| بيانات النشر: | BMC |
| سنة النشر: | 2008 |
| المجموعة: | Directory of Open Access Journals: DOAJ Articles |
| مصطلحات موضوعية: | Microbiology, QR1-502 |
| الوصف: | Background Microbiological indicators are commonly used in the assessment of public health risks associated with fecal contamination of freshwater ecosystems. Sediments are a reservoir of microorganisms, and can thus provide information on past pollution events, not obtainable through the testing of surface water. Moreover, pathogens present in sediment may represent future threats to human health. Clostridium perfringens , a typical colonizer of sediments, has been suggested as an alternative indicator of fecal pollution. In order to be suitable for such purpose, the microorganism should be widely distributed in contaminated environments. The objective of this study was thus to determine the composition of the anaerobic community in sediment samples of the lower Tiber basin, in central Italy, through a combined approach involving granulometric analysis of sediment samples, as well as a microbiological and molecular (16S rRNA) analysis of strains. Results Granulometry showed a similar, clayey sediment composition, in most sampling sites. The microbiological method, employing, an adaptation of the standard method, proved to be effective in isolating anaerobic bacteria from the environmental matrix for the purpose of genetic analysis. Eighty-three strains of bacteria were isolated and the partial 16S rRNA gene sequenced. While biochemical analysis detected only C. perfringens strains, phylogenetic analysis indicated the presence of three clusters: C. perfringens, C. bifermentans and B. cereus , comprising eight taxa. C. perfringens , the commonest in almost all sediment sampling sites, was present in all sites, and in both seasons (seasonal sampling was carried out only along the Tiber and Aniene rivers). None of the described genetic profiles showed complete similarity with GenBank sequences. Conclusion The study underlines the value of C. perfringens as an alternative microbial indicator of fecal contamination in river sediments. This is supported by the bacterium's presence in all sampling sites, and ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 1471-2180 |
| العلاقة: | http://www.biomedcentral.com/1471-2180/8/171Test; https://doaj.org/toc/1471-2180Test; https://doaj.org/article/d0b70401875046b59fd2796cef2bc2f8Test |
| DOI: | 10.1186/1471-2180-8-171 |
| الإتاحة: | https://doi.org/10.1186/1471-2180-8-171Test https://doaj.org/article/d0b70401875046b59fd2796cef2bc2f8Test |
| رقم الانضمام: | edsbas.4CB69439 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 14712180 |
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| DOI: | 10.1186/1471-2180-8-171 |