دورية أكاديمية
Tricking enzymes in living cells: a mechanism-based strategy for design of DNA topoisomerase biosensors
| العنوان: | Tricking enzymes in living cells: a mechanism-based strategy for design of DNA topoisomerase biosensors |
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| المؤلفون: | Sai Ba, Guangpeng Gao, Tianhu Li, Hao Zhang |
| المصدر: | Journal of Nanobiotechnology, Vol 19, Iss 1, Pp 1-14 (2021) |
| بيانات النشر: | BMC, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Biotechnology LCC:Medical technology |
| مصطلحات موضوعية: | DNA topoisomerases, Cancer diagnosis, Enzyme mechanisms, DNA nanotechnology, Diagnostic probes, Biotechnology, TP248.13-248.65, Medical technology, R855-855.5 |
| الوصف: | Abstract Most activity-based molecular probes are designed to target enzymes that catalyze the breaking of chemical bonds and the conversion of a unimolecular substrate into bimolecular products. However, DNA topoisomerases are a class of enzymes that alter DNA topology without producing any molecular segments during catalysis, which hinders the development of practical methods for diagnosing these key biomarkers in living cells. Here, we established a new strategy for the effective sensing of the expression levels and catalytic activities of topoisomerases in cell-free systems and human cells. Using our newly designed biosensors, we tricked DNA topoisomerases within their catalytic cycles to switch on fluorescence and resume new rounds of catalysis. Considering that human topoisomerases have been widely recognized as biomarkers for multiple cancers and identified as promising targets for several anticancer drugs, we believe that these DNA-based biosensors and our design strategy would greatly benefit the future development of clinical tools for cancer diagnosis and treatment. Graphical Abstract |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1477-3155 |
| العلاقة: | https://doaj.org/toc/1477-3155Test |
| DOI: | 10.1186/s12951-021-01155-1 |
| الوصول الحر: | https://doaj.org/article/5b6187f821af420d8a499c8293faa5cfTest |
| رقم الانضمام: | edsdoj.5b6187f821af420d8a499c8293faa5cf |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14773155 |
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| DOI: | 10.1186/s12951-021-01155-1 |