دورية أكاديمية
Metabolic engineering of Escherichia coli for direct production of vitamin C from D-glucose
العنوان: | Metabolic engineering of Escherichia coli for direct production of vitamin C from D-glucose |
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المؤلفون: | Yong-Sheng Tian, Yong-Dong Deng, Wen-Hui Zhang, Yu-Wang, Jing Xu, Jian-Jie Gao, Bo-Wang, Xiao-Yan Fu, Hong-Juan Han, Zhen-Jun Li, Li-Juan Wang, Ri-He Peng, Quan-Hong Yao |
المصدر: | Biotechnology for Biofuels and Bioproducts, Vol 15, Iss 1, Pp 1-13 (2022) |
بيانات النشر: | BMC, 2022. |
سنة النشر: | 2022 |
المجموعة: | LCC:Biotechnology LCC:Fuel |
مصطلحات موضوعية: | Vitamin C, One-step fermentation, D-Glucose, Synthesis pathway, Biotechnology, TP248.13-248.65, Fuel, TP315-360 |
الوصف: | Abstract Background Production of vitamin C has been traditionally based on the Reichstein process and the two-step process. However, the two processes share a common disadvantage: vitamin C cannot be directly synthesized from D-glucose. Therefore, significant effort has been made to develop a one-step vitamin C fermentation process. While, 2-KLG, not vitamin C, is synthesized from nearly all current one-step fermentation processes. Vitamin C is naturally synthesized from glucose in Arabidopsis thaliana via a ten-step reaction pathway that is encoded by ten genes. The main objective of this study was to directly produce vitamin C from D-glucose in Escherichia coli by expression of the genes from the A. thaliana vitamin C biosynthetic pathway. Results Therefore, the ten genes of whole vitamin C synthesis pathway of A. thaliana were chemically synthesized, and an engineered strain harboring these genes was constructed in this study. The direct production of vitamin C from D-glucose based on one-step fermentation was achieved using this engineered strain and at least 1.53 mg/L vitamin C was produced in shaking flasks. Conclusions The study demonstrates the feasibility of one-step fermentation for the production of vitamin C from D-glucose. Importantly, the one-step process has significant advantages compared with the currently used fermentation process: it can save multiple physical and chemical steps needed to convert D-glucose to D-sorbitol; it also does not involve the associated down-streaming steps required to convert 2-KLG into vitamin C. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 2731-3654 |
العلاقة: | https://doaj.org/toc/2731-3654Test |
DOI: | 10.1186/s13068-022-02184-0 |
الوصول الحر: | https://doaj.org/article/59e18f865c4b47229bf522dbb7b2c20dTest |
رقم الانضمام: | edsdoj.59e18f865c4b47229bf522dbb7b2c20d |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 27313654 |
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DOI: | 10.1186/s13068-022-02184-0 |