دورية أكاديمية
Physiological and biochemical characterization of egg extract of black widow spiders to uncover molecular basis of egg toxicity
| العنوان: | Physiological and biochemical characterization of egg extract of black widow spiders to uncover molecular basis of egg toxicity |
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| المؤلفون: | Yizhong Yan, Jianjun Li, Yiya Zhang, Xiaozhen Peng, Tianyao Guo, Jirong Wang, Weijun Hu, Zhigui Duan, Xianchun Wang |
| المصدر: | Biological Research, Vol 47, Iss 0, Pp 1-11 (2014) |
| بيانات النشر: | BMC, 2014. |
| سنة النشر: | 2014 |
| المجموعة: | LCC:Biology (General) |
| مصطلحات موضوعية: | Black widow spider (L. tredecimguttatus), Protein composition, Egg, Molecular mechanism, Property analysis, Toxicity, Biology (General), QH301-705.5 |
| الوصف: | BACKGROUND: Black widow spider (L. tredecimguttatus) has toxic components not only in the venomous glands, but also in other parts of the body and its eggs. It is biologically important to investigate the molecular basis of the egg toxicity. RESULTS: In the present work, an aqueous extract was prepared from the eggs of the spider and characterized using multiple physiological and biochemical strategies. Gel electrophoresis and mass spectrometry demonstrated that the eggs are rich in high-molecular-mass proteins and the peptides below 5 kDa. The lyophilized extract of the eggs had a protein content of 34.22% and was shown to have a strong toxicity towards mammals and insects. When applied at a concentration of 0.25 mg/mL, the extract could completely block the neuromuscular transmission in mouse isolated phrenic nerve-hemidiaphragm preparations within 12.0 ± 1.5 min. Using whole-cell patch-clamp technique, the egg extract was demonstrated to be able to inhibit the voltage-activated Na+, K+and Ca2+ currents in rat DRG neurons. In addition, the extract displayed activities of multiple hydrolases. Finally, the molecular basis of the egg toxicity was discussed. CONCLUSIONS: The eggs of black widow spiders are rich in proteinous compounds particularly the high-molecular-mass proteins with different types of biological activity The neurotoxic and other active compounds in the eggs are believed to play important roles in the eggs' toxic actions. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0716-9760 0717-6287 91238943 |
| العلاقة: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100017&lng=en&tlng=enTest; https://doaj.org/toc/0716-9760Test |
| DOI: | 10.1186/0717-6287-47-17 |
| الوصول الحر: | https://doaj.org/article/914e73c384f9432c912389437bca67d6Test |
| رقم الانضمام: | edsdoj.914e73c384f9432c912389437bca67d6 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 07169760 07176287 91238943 |
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| DOI: | 10.1186/0717-6287-47-17 |