Physiological and biochemical characterization of egg extract of black widow spiders to uncover molecular basis of egg toxicity
| العنوان: | Physiological and biochemical characterization of egg extract of black widow spiders to uncover molecular basis of egg toxicity |
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| المؤلفون: | Yiya Zhang, Tianyao Guo, Zhigui Duan, Jirong Wang, Weijun Hu, Xiaozhen Peng, Yizhong Yan, Xianchun Wang, Jianjun Li |
| المصدر: | Biological Research, Vol 47, Iss 0, Pp 1-11 (2014) Biological Research Biological Research, Volume: 47, Pages: 1-11, Published: 2014 Biological Research v.47 2014 SciELO Chile CONICYT Chile instacron:CONICYT |
| بيانات النشر: | BMC, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Black widow spider (L. tredecimguttatus), Neuromuscular transmission, Cockroaches, Voltage-Gated Sodium Channels, Arthropod Proteins, Molecular mechanism, Toxicology, Mice, Animal Shells, biology.animal, Ganglia, Spinal, Animals, Black Widow Spider, Egg, Protein composition, lcsh:QH301-705.5, Ovum, Medicine(all), Gel electrophoresis, Spider, Cockroach, Agricultural and Biological Sciences(all), biology, Toxicity, Biochemistry, Genetics and Molecular Biology(all), Tissue Extracts, Biological activity, Rats, Phrenic Nerve, Biochemistry, lcsh:Biology (General), Potassium Channels, Voltage-Gated, embryonic structures, Property analysis, Calcium Channels, Developmental biology, Research Article |
| الوصف: | BACKGROUND: Black widow spider (L. tredecimguttatus) has toxic components not only in the venomous glands, but also in other parts of the body and its eggs. It is biologically important to investigate the molecular basis of the egg toxicity. RESULTS: In the present work, an aqueous extract was prepared from the eggs of the spider and characterized using multiple physiological and biochemical strategies. Gel electrophoresis and mass spectrometry demonstrated that the eggs are rich in high-molecular-mass proteins and the peptides below 5 kDa. The lyophilized extract of the eggs had a protein content of 34.22% and was shown to have a strong toxicity towards mammals and insects. When applied at a concentration of 0.25 mg/mL, the extract could completely block the neuromuscular transmission in mouse isolated phrenic nerve-hemidiaphragm preparations within 12.0 ± 1.5 min. Using whole-cell patch-clamp technique, the egg extract was demonstrated to be able to inhibit the voltage-activated Na+, K+and Ca2+ currents in rat DRG neurons. In addition, the extract displayed activities of multiple hydrolases. Finally, the molecular basis of the egg toxicity was discussed. CONCLUSIONS: The eggs of black widow spiders are rich in proteinous compounds particularly the high-molecular-mass proteins with different types of biological activity The neurotoxic and other active compounds in the eggs are believed to play important roles in the eggs' toxic actions. |
| وصف الملف: | text/html |
| اللغة: | English |
| تدمد: | 0716-9760 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9e4d75181f16584fedfc7d10f80b3c0eTest http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602014000100017&lng=en&tlng=enTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9e4d75181f16584fedfc7d10f80b3c0e |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 07169760 |
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