Long non-coding RNA PART1 predicts a poor prognosis and promotes the malignant progression of pancreatic cancer by sponging miR-122
| العنوان: | Long non-coding RNA PART1 predicts a poor prognosis and promotes the malignant progression of pancreatic cancer by sponging miR-122 |
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| المؤلفون: | Junhong Ma, Jingjing Tian, Xibao Hu, Lei Zhang |
| المصدر: | World Journal of Surgical Oncology, Vol 19, Iss 1, Pp 1-11 (2021) World Journal of Surgical Oncology |
| بيانات النشر: | BMC, 2021. |
| سنة النشر: | 2021 |
| مصطلحات موضوعية: | Male, 0301 basic medicine, PART1, RNA, Untranslated, RD1-811, 03 medical and health sciences, 0302 clinical medicine, Surgical oncology, Sponge, Cell Line, Tumor, Pancreatic cancer, MiR-122, Humans, Medicine, RC254-282, Cell Proliferation, Gene knockdown, Progression, business.industry, Cell growth, Research, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Prognosis, medicine.disease, Long non-coding RNA, miR-122, Gene Expression Regulation, Neoplastic, Pancreatic Neoplasms, MicroRNAs, 030104 developmental biology, Oncology, Apoptosis, 030220 oncology & carcinogenesis, Androgens, Disease Progression, Cancer research, RNA, Long Noncoding, Surgery, business |
| الوصف: | Background and objectives Long non-coding RNA (lncRNA) prostate androgen-regulated transcript 1 (PART1) was previously shown to exert an oncogenic role in several human cancers. However, whether PART1 is associated with the malignant progression of pancreatic cancer remains unclear. In the current study, we aimed to identify the role and potential mechanism of PART1 in pancreatic cancer. Methods qRT-PCR was applied to detect PART1 expression in 45 cases of pancreatic cancer patients. The chi-square test was performed to assess the association between PART1 expression and clinicopathologic features, and Kaplan-Meier method was applied to evaluate overall survival. In vitro CCK-8, transwell invasion, and flow cytometry assays were applied to detect the effects of PART1 on cell proliferation, invasion, and apoptosis, respectively. Luciferase reporter and RNA immunoprecipitation assays were used to identify the regulatory mechanism between PART1 and miR-122. Results PART1 expression was upregulated in pancreatic cancer tissues and cell lines. High PART1 expression was closely correlated with tumor size, T classification, clinical stage, and vascular invasion, and predicted a poor overall survival. PART1 knockdown significantly suppressed cell proliferation and invasion abilities of pancreatic cancer but promoted cell apoptosis. PART1 was found to serve as a molecular sponge of miR-122, and miR-122 inhibition partially reversed the inhibitory phenotypes of PART1 knockdown on pancreatic cancer cells. Conclusions PART1 promotes the malignant progression of pancreatic cancer by sponging miR-122. The PART1/miR-122 axis might be a promising target for anticancer therapy in patients with pancreatic cancer. |
| اللغة: | English |
| تدمد: | 1477-7819 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::91c0a146cfd12c695ef48893ebde4c13Test https://doaj.org/article/62fd8267d6384bbfa583c86eb46e681dTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....91c0a146cfd12c695ef48893ebde4c13 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14777819 |
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