Comprehensive microRNA profiling reveals potential augmentation of the IL1 pathway in rheumatic heart valve disease
| العنوان: | Comprehensive microRNA profiling reveals potential augmentation of the IL1 pathway in rheumatic heart valve disease |
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| المؤلفون: | Yi Sun, Qiyu Lu, Yuyin Duan, Bin Li, Guimin Zhang, Songhua Yu, Jianming Xia |
| المصدر: | BMC Cardiovascular Disorders, Vol 18, Iss 1, Pp 1-12 (2018) BMC Cardiovascular Disorders |
| بيانات النشر: | BMC, 2018. |
| سنة النشر: | 2018 |
| مصطلحات موضوعية: | Adult, Genetic Markers, Male, 0301 basic medicine, Pathology, medicine.medical_specialty, lcsh:Diseases of the circulatory (Cardiovascular) system, Heart disease, medicine.medical_treatment, Interleukin-1beta, 030204 cardiovascular system & hematology, Interleukin 1, 03 medical and health sciences, 0302 clinical medicine, Mitral valve stenosis, Mitral valve, medicine, Humans, Mitral valve prolapse, Circulating MicroRNA, Heart valve, Rheumatic heart disease, Aged, miRNA, Receptors, Interleukin-1 Type I, Inflammation, Mitral Valve Prolapse, Reverse Transcriptase Polymerase Chain Reaction, business.industry, Gene Expression Profiling, valvular heart disease, Mitral valve replacement, Mitral Valve Insufficiency, Middle Aged, medicine.disease, Valvular heart disease, MicroRNAs, HEK293 Cells, 030104 developmental biology, medicine.anatomical_structure, lcsh:RC666-701, Case-Control Studies, Mitral Valve, Rheumatic fever, Female, Cardiology and Cardiovascular Medicine, business, Signal Transduction, Research Article |
| الوصف: | Background Valvular heart disease is a leading cause of cardiovascular mortality, especially in China. More than a half of valvular heart diseases are caused by acute rheumatic fever. microRNA is involved in many physiological and pathological processes. However, the miRNA profile of the rheumatic valvular heart disease is unknown. This research is to discuss microRNAs and their target gene pathways involved in rheumatic heart valve disease. Methods Serum miRNA from one healthy individual and four rheumatic heart disease patients were sequenced. Specific differentially expressed miRNAs were quantified by Q-PCR in 40 patients, with 20 low-to-moderate rheumatic mitral valve stenosis patients and 20 severe mitral valve stenosis patients. The target relationship between certain miRNA and predicted target genes were analysis by Luciferase reporter assay. The IL-1β and IL1R1 expression levels were analyzed by immunohistochemistry and western blot in the mitral valve from surgery of mitral valve replacement. Results The results showed that 13 and 91 miRNAs were commonly upregulated or downregulated in all four patients. Nine miRNAs, 1 upregulated and 8 downregulated, that had a similar fold change in all 4 patients were selected for quantitative PCR verification. The results showed similar results from miRNA sequencing. Within these 9 tested miRNAs, hsa-miR-205-3p and hsa-miR-3909 showed a low degree of dispersion between the members of each group. Hsa miR-205-3p and hsa-miR-3909 were predicted to target the 3’UTR of IL-1β and IL1R1 respectively. This was verified by luciferase reporter assays. Immunohistochemistry and Western blot results showed that the mitral valve from rheumatic valve heart disease showed higher levels of IL- 1β and IL1R1 expression compared with congenital heart valve disease. This suggested a difference between rheumatic heart valve disease and other types of heart valve diseases, with more inflammatory responses in the former. Conclusion In the present study, by next generation sequencing of miRNAs, it was revealed that interleukin 1β and interleukin 1 receptor 1 was involved in rheumatic heart diseases. And this is useful for diagnosis and understanding of mechanism of rheumatic heart disease. Electronic supplementary material The online version of this article (10.1186/s12872-018-0788-2) contains supplementary material, which is available to authorized users. |
| اللغة: | English |
| تدمد: | 1471-2261 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::86232bb6809ca1dcbf106e8d5173d521Test http://link.springer.com/article/10.1186/s12872-018-0788-2Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....86232bb6809ca1dcbf106e8d5173d521 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14712261 |
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