DNA recovery from archived RDTs for genetic characterization of Plasmodium falciparum in a routine setting in Lambaréné, Gabon
| العنوان: | DNA recovery from archived RDTs for genetic characterization of Plasmodium falciparum in a routine setting in Lambaréné, Gabon |
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| المؤلفون: | Nguyen, The Trong, Nzigou Mombo, Brice, Lalremruata, Albert, Koehne, Erik, Zoleko Manego, Rella, Dimessa Mbadinga, Lia Betty, Adegnika, Ayola Akim, Agnandji, Selidji Todagbe, Lell, Bertrand, Kremsner, Peter Gottfried, Velavan, Thirumalaisamy P, Ramharter, Michael, Mordmüller, Benjamin, Mombo-Ngoma, Ghyslain |
| المصدر: | Malaria Journal, Vol 18, Iss 1, Pp 1-10 (2019) Malaria Journal |
| بيانات النشر: | BMC, 2019. |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Adult, Male, lcsh:Arctic medicine. Tropical medicine, Adolescent, Genotype, lcsh:RC955-962, Plasmodium falciparum, Drug Resistance, Protozoan Proteins, Parasitemia, pfcrt, Body Temperature, lcsh:Infectious and parasitic diseases, Capillary electrophoresis, Young Adult, parasitic diseases, Humans, lcsh:RC109-216, Gabon, Malaria, Falciparum, Child, RDT, Merozoite Surface Protein 1, Biological Specimen Banks, Retrospective Studies, Research, Membrane Transport Proteins, Chloroquine, DNA, Protozoan, Malaria, Molecular Diagnostic Techniques, Child, Preschool, msp1, Female |
| الوصف: | Background Rapid diagnostic tests (RDTs) have been described as a source of genetic material to analyse malaria parasites in proof-of-concept studies. The increasing use of RDTs (e.g., in focal or mass screening and treatment campaigns) makes this approach particularly attractive for large-scale investigations of parasite populations. In this study, the complexity of Plasmodium falciparum infections, parasite load and chloroquine resistance transporter gene mutations were investigated in DNA samples extracted from positive RDTs, obtained in a routine setting and archived at ambient temperature. Methods A total of 669 archived RDTs collected from malaria cases in urban, semi-urban and rural areas of central Gabon were used for P. falciparum DNA extraction. Performance of RDTs as a source of DNA for PCR was determined using: (i) amplification of a single copy merozoite surface protein 1 (msp1) gene followed by highly sensitive and automated capillary electrophoresis; (ii) genotyping of the pfcrt gene locus 72–76 using haplotype-specific-probe-based real-time PCR to characterize chloroquine resistance; and, (iii) real-time PCR targeting 18S genes to detect and quantify Plasmodium parasites. Results Out of the 669 archived RDTs, amplification of P. falciparum nucleic materials had a success rate of 97% for 18S real-time PCR, and 88% for the msp1 gene. The multiplicity of infections (MOI) of the whole population was 2.6 (95% CI 2.5–2.8). The highest number of alleles detected in one infection was 11. The MOI decreased with increasing age (β = − 0.0046, p = 0.02) and residence in Lambaréné was associated with smaller MOIs (p |
| اللغة: | English |
| تدمد: | 1475-2875 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=pmid_dedup__::431146d06f5f004aa7bdb6147351c00bTest http://link.springer.com/article/10.1186/s12936-019-2972-yTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.pmid.dedup....431146d06f5f004aa7bdb6147351c00b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14752875 |
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