مورد إلكتروني
Signalling organelle for retrograde axonal transport of internalized neurotrophins from the nerve terminal
| العنوان: | Signalling organelle for retrograde axonal transport of internalized neurotrophins from the nerve terminal |
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| المصدر: | Immunology and Cell Biology |
| بيانات النشر: | Blackwell Publishing Ltd 2000 |
| تفاصيل مُضافة: | Sandow, Shaun L Heydon, Katharina Weible, Michael Reynolds, Anna Bartlett, Selena Hendry, Ian |
| نوع الوثيقة: | Electronic Resource |
| مستخلص: | The retrograde axonal transport of neurotrophins occurs after receptor-mediated endocytosis into vesicles at the nerve terminal. We have been investigating the process of targeting these vesicles for retrograde transport, by examining the transport of [125I]-labelled neurotrophins from the eye to sympathetic and sensory ganglia. With the aid of confocal microscopy, we examined the phenomena further in cultures of dissociated I sympathetic ganglia to which rhodamine-labelled nerve growth factor (NGF) was added. We found the label in large vesicles in the growth cone and axons. Light microscopic examination of the sympathetic nerve trunk in vivo also showed the retrogradely transported material to be sporadically located in large structures in the axons. Ultra-structural examination of the sympathetic nerve trunk after the transport of NGF bound to gold particles showed the label to be concentrated in relatively few large organelles that consisted of accumulations of multivesicular bodies. These results suggest that in vivo NGF is transported in specialized organelles that require assembly in the nerve terminal. |
| مصطلحات الفهرس: | Keywords: iodine 125; nerve growth factor; neurotrophin; animal cell; conference paper; confocal microscopy; electron microscopy; mouse; nerve cell; nerve ending; nerve fiber transport; newborn; nonhuman; signal transduction; Animals; Animals, Newborn; Axonal Trans Confocal microscopy; Electron microscopy; Nerve growth factor; Retrograde axonal transport; Vesicle recycling, Journal article |
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| الإتاحة: | Open access content. Open access content |
| أرقام أخرى: | AUANP oai:openresearch-repository.anu.edu.au:1885/34595 0818-9641 1291768068 |
| المصدر المساهم: | AUSTRALIAN NAT UNIV - PRINT REPOSITORY From OAIster®, provided by the OCLC Cooperative. |
| رقم الانضمام: | edsoai.on1291768068 |
| قاعدة البيانات: | OAIster |
| الوصف غير متاح. |