Type-specific dysregulation of matrix metalloproteinases and their tissue inhibitors in end-stage heart failure patients: relationship between MMP-10 and LV remodelling
| العنوان: | Type-specific dysregulation of matrix metalloproteinases and their tissue inhibitors in end-stage heart failure patients: relationship between MMP-10 and LV remodelling |
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| المؤلفون: | Chuan-Jue Cui, Xiaoling Zhang, Jun Li, Hao Zhang, Shengshou Hu, Mitja Lainscak, Zhe Zheng, Jie Huang, Yingjie Wei |
| المصدر: | Journal of Cellular and Molecular Medicine |
| بيانات النشر: | Blackwell Publishing Ltd, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Cardiomyopathy, Dilated, Male, medicine.medical_specialty, Adolescent, Hemodynamics, Enzyme-Linked Immunosorbent Assay, Matrix metalloproteinase, metalloproteinases, Western blot, Matrix Metalloproteinase 10, Insulin-Like Growth Factor II, Internal medicine, medicine, Animals, Humans, Ventricular remodeling, Child, Heart Failure, medicine.diagnostic_test, Ventricular Remodeling, business.industry, Myocardium, Reproducibility of Results, Dilated cardiomyopathy, Tissue Inhibitor of Metalloproteinases, Cell Biology, Articles, Middle Aged, medicine.disease, Rats, Endocrinology, left ventricular remodelling, Heart failure, Matrix Metalloproteinase 7, Cohort, Molecular Medicine, Immunohistochemistry, Female, business |
| الوصف: | Although past studies observed the changes of matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) in end-stage heart failure (HF) patients, a consistent and clear pattern of type-specific MMPs and/or TIMPs has yet to be further defined. In this study, proteomic approach of human protein antibody arrays was used to compare MMP and TIMP expression levels of left ventricular (LV) myocardial samples from end-stage HF patients due to dilated cardiomyopathy (DCM) with those from age- and sex- matched non-failing patients. Western blot analysis, immunohistochemistry and ELISA were used for validation of our results. We observed that MMP-10 and -7 abundance increased, accompanied by decreased TIMP-4 in DCM failing hearts (n= 8) compared with non-failing hearts (n= 8). The results were further validated in a cohort of 34 end-stage HF patients derived from three forms of cardiomyopathies. Cardiac and plasma MMP-10 levels were positively correlated with the LV end-diastolic dimension in this HF cohort. In addition, we observed that insulin-like growth factor-2 promoted MMP-10 production in neonatal rat cardiomyocytes. In conclusion, this study demonstrated a selective up-regulation of MMP-10 and -7 along with a discordant change of TIMP-4, and a positive correlation between MMP-10 levels and the degree of LV dilation in end-stage HF patients. Our findings suggest that type-specific dysregulation of MMPs and TIMPs is associated with LV remodelling in end-stage HF patients, and MMP-10 may act as a novel biomarker for LV remodelling. |
| اللغة: | English |
| تدمد: | 1582-4934 1582-1838 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::29e8d27ef507b003e752ff666854d516Test http://europepmc.org/articles/PMC3922666Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....29e8d27ef507b003e752ff666854d516 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15824934 15821838 |
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