The plaque protein myozap identified as a novel major component of adhering junctions in endothelia of the blood and the lymph vascular systems
| العنوان: | The plaque protein myozap identified as a novel major component of adhering junctions in endothelia of the blood and the lymph vascular systems |
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| المؤلفون: | Stefanie Winter-Simanowski, Christian Kuhn, Caecilia Kuhn, Sebastian Pieperhoff, Hans Heid, Ralf Zimbelmann, Werner W. Franke, Steffen Rickelt, Norbert Frey, William C. Claycomb |
| المصدر: | Journal of Cellular and Molecular Medicine Pieperhoff, S, Rickelt, S, Heid, H, Claycomb, W C, Zimbelmann, R, Kuhn, C, Winter-Simanowski, S, Kuhn, C, Frey, N & Franke, W W 2012, ' The plaque protein myozap identified as a novel major component of adhering junctions in endothelia of the blood and the lymph vascular systems ', Journal of Cellular and Molecular Medicine, vol. 16, no. 8, pp. 1709-1719 . https://doi.org/10.1111/j.1582-4934.2011.01463.xTest |
| بيانات النشر: | Blackwell Publishing Ltd, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Pathology, medicine.medical_specialty, Endothelium, Immunoprecipitation, Immunoblotting, Sus scrofa, Plakoglobin, Fluorescent Antibody Technique, Cell Line, Adherens junction, blood vessels, lymphatic vessels, Mice, Antibody Specificity, medicine, Animals, Humans, Myocytes, Cardiac, biology, Desmoplakin, Myocardium, Endothelial Cells, Membrane Proteins, Cell Biology, Plectin, Original Articles, Adherens Junctions, Transmembrane protein, Cell biology, Rats, Pulmonary Alveoli, medicine.anatomical_structure, biology.protein, Molecular Medicine, Cattle, Electrophoresis, Polyacrylamide Gel, Lymph, Endothelium, Vascular, myozap |
| الوصف: | Recently the protein myozap (myocardium-enriched zonula adhaerens protein), a 54-kDa polypeptide, which is not a member of any of the known cytoskeletal and junctional protein multigene families, has been identified as a constituent of the plaques of the composite junctions in the intercalated disks connecting the cardiomyocytes of mammalian hearts. Using a set of novel, highly sensitive and specific antibodies we now report that myozap is also a major constituent of the cytoplasmic plaques of the adherens junctions (AJs) connecting the endothelial cells of the mammalian blood and lymph vascular systems, including the desmoplakin-containing complexus adhaerentes of the virgultar cells of lymph node sinus. In light and electron microscopic immunolocalization experiments we show that myozap colocalizes with several proteins of desmosomal plaques as well as with AJ-specific transmembrane molecules, including VE-cadherin. In biochemical analyses, rigorous immunoprecipitation experiments have revealed N-cadherin, desmoplakin, desmoglein-2, plakophilin-2, plakoglobin and plectin as very stably bound complex partners. We conclude that myozap is a general component of cell-cell junctions not only in the myocardium but also in diverse endothelia of the blood and lymph vascular systems of adult mammals, suggesting that this protein not only serves a specific role in the heart but also a broader set of functions in the vessel systems. We also propose to use myozap as an endothelial cell type marker in diagnoses. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1582-4934 1582-1838 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3bea63c84aa94fa0cceed12a9a2e5c7fTest http://europepmc.org/articles/PMC3822684Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3bea63c84aa94fa0cceed12a9a2e5c7f |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15824934 15821838 |
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