Adipose-derived stem cells accelerate neovascularization in ischaemic diabetic skin flap via expression of hypoxia-inducible factor-1α
| العنوان: | Adipose-derived stem cells accelerate neovascularization in ischaemic diabetic skin flap via expression of hypoxia-inducible factor-1α |
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| المؤلفون: | Lei Cui, Weicheng Gao, Shaolin Ma, Xing Qiao |
| المصدر: | Journal of Cellular and Molecular Medicine |
| بيانات النشر: | Blackwell Publishing Ltd, 2011. |
| سنة النشر: | 2011 |
| مصطلحات موضوعية: | Male, Vascular Endothelial Growth Factor A, Pathology, skin flaps, Adipose tissue, Apoptosis, Surgical Flaps, Neovascularization, Immunoenzyme Techniques, chemistry.chemical_compound, Mice, Cell Movement, Ischemia, Adipocytes, Hypoxia, Cells, Cultured, Mice, Inbred BALB C, Neovascularization, Pathologic, Stem Cells, Graft Survival, Skin Transplantation, Flow Cytometry, Vascular endothelial growth factor, Vascular endothelial growth factor A, medicine.anatomical_structure, diabetes mellitus, Molecular Medicine, medicine.symptom, Stem cell, Adult, medicine.medical_specialty, Blotting, Western, Enzyme-Linked Immunosorbent Assay, Biology, Diabetes Mellitus, Experimental, Dermis, medicine, Cell Adhesion, Animals, Humans, Cell Proliferation, Cell Biology, Original Articles, Hypoxia-Inducible Factor 1, alpha Subunit, eye diseases, Surgery, chemistry, adipose-derived stem cells, Ex vivo |
| الوصف: | Skin flaps are frequently performed for diabetic patients in spite of countless detrimental effects of diabetes on flap survival, most of which may result from a defective response of the tissues to low oxygen tension. In this study, the authors explored the feasibility of applying human adipose-derived stem cells (ASCs) to increase the viability of random-patterned skin flaps in streptozotocin-induced diabetic mice. ASCs were isolated from the fresh human lipoaspirates and expanded ex vivo for three passages. After the elevation of caudally based random-patterned skin flaps (3 cm long and 1 cm wide), ASCs suspensions were then injected into the flap (group A). Media containing no ASCs were similarly injected as a control (group B), although nothing was injected into the flap base of mice in control group C. Flap assessments were carried out at post-operative day 7 for evaluation of flap viability. The flap survival rate of group A was significantly higher than those of groups B and C, whereas no difference was observed between groups B and C. Histological examination also demonstrated a statistically significant increase in capillary density in group A over both groups B and C. Furthermore, it was found that ASCs not only augmented the expression of vascular endothelial growth factor and hypoxia-inducible factor-1α (HIF-1α) in flap tissues from dermis of diabetes mice, but also promoted their expression in dermal fibroblasts from diabetic mice. Thus, ASCs could enhance the survival of random-patterned skin flaps in streptozotocin-induced diabetic mice via elevated expression of HIF-1α. |
| اللغة: | English |
| تدمد: | 1582-4934 1582-1838 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::dd8f5477c224871bf2da039d60eb9912Test http://europepmc.org/articles/PMC4373426Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....dd8f5477c224871bf2da039d60eb9912 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15824934 15821838 |
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