دورية أكاديمية
Elastic fiber proteins in the glomerular mesangium in vivo and in cell culture
| العنوان: | Elastic fiber proteins in the glomerular mesangium in vivo and in cell culture |
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| المؤلفون: | Sterzel, R., Hartner, A., Schlotzer-Schrehardt, U., Voit, S., Hausknecht, B., Doliana, R., Colombatti, A., Gibson, M., Braghetta, P., Bressan, G. |
| بيانات النشر: | Blackwell Publishing Inc |
| سنة النشر: | 2000 |
| المجموعة: | The University of Adelaide: Digital Library |
| مصطلحات موضوعية: | Glomerular Mesangium, Microcirculation, Cells, Cultured, Extracellular Matrix, Epithelial Cells, Animals, Humans, Mice, Rats, Elastin, Microfilament Proteins, Platelet-Derived Growth Factor, Proto-Oncogene Proteins c-sis, Transforming Growth Factor beta, Intracellular Signaling Peptides and Proteins, Carrier Proteins, Contractile Proteins, Membrane Glycoproteins, Extracellular Matrix Proteins, RNA, Messenger, Anticoagulants, Microscopy, Immunoelectron, Fluorescent Antibody Technique, Gene Expression, Homeostasis, Elasticity, Hydrostatic Pressure |
| الوصف: | Background Glomerular capillaries of the mammalian kidney are exposed to high intraluminal hydrostatic pressures and require elastic constraint to maintain size, shape, and integrity. Previous morphological and functional studies indicated that the extracellular matrices of glomeruli, that is, basement membrane and mesangial matrix, contribute to glomerular resilience and mechanical stability. Immunofluorescence microscopy findings demonstrated elastic fiber components to be located in the renal vasculature, including glomeruli. The aim of this study was to clarify the exact glomerular localization, composition, and cellular production of these proteins. Methods We examined the renal distribution of the elastic fiber proteins fibrillin-1, emilin, microfibril-associated glycoproteins (MAGPs) 1 and 2, latent transforming growth factor-binding protein-1 (LTBP-1), and elastin using immunohistology and immunoelectron microscopy of human, rat, and mouse kidneys. In mesangial cell cultures, we also studied the expression and extracellular deposition of such proteins by use of Northern blotting and immunocytochemistry. Results Fibrillin-1, emilin, MAGPs 1 and 2, and LTBP-1 were present in glomeruli of mouse, rat, and human kidney, where they were located predominantly in the mesangial extracellular matrix underlying glomerular endothelium and basement membrane. Several of these proteins, as well as elastin, were also expressed in the renal vasculature. While elastin localized to the glomerular vascular pole in afferent and efferent arterioles extending to Bowman's capsule, it was not found in the glomerular capillary tuft. Cultured mesangial cells of rat, mouse, and human kidneys expressed mRNAs of fibrillin-1, emilin, MAGP-2, and elastin, and the respective proteins localized within and outside of mesangial cells, as shown by immunocytochemistry. mRNA expression of fibrillin-1, emilin, and elastin was strong in quiescent mesangial cells; their gene expression was further up-regulated by transforming growth ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 0085-2538 1523-1755 |
| العلاقة: | Kidney International, 2000; 58(4):1588-1602; http://hdl.handle.net/2440/5763Test |
| DOI: | 10.1046/j.1523-1755.2000.00320.x |
| الإتاحة: | https://doi.org/10.1046/j.1523-1755.2000.00320.xTest http://hdl.handle.net/2440/5763Test |
| رقم الانضمام: | edsbas.B62DB438 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 00852538 15231755 |
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| DOI: | 10.1046/j.1523-1755.2000.00320.x |