دورية أكاديمية
Production and release of heat-labile toxin by wild-type human-derived enterotoxigenic Escherichia coli
| العنوان: | Production and release of heat-labile toxin by wild-type human-derived enterotoxigenic Escherichia coli |
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| المؤلفون: | Simoes Lasaro, Melissa Ang, Rodrigues, Juliana Falcao, Mathias-Santos, Camila, Guth, Beatriz Ernestina Cabilio UNIFESP, Regua-Mangia, Adriana, Piantino Ferreira, Antonio Jose, Takagi, Mickie, Cabrera-Crespo, Joaquin, Sbrogio-Almeida, Maria Elisabete, Souza Ferreira, Luis Carlos de |
| المساهمون: | Universidade de São Paulo (USP), Universidade Federal de São Paulo (UNIFESP), Fdn Oswaldo Cruz, Inst Butantan |
| بيانات النشر: | Blackwell Publishing |
| سنة النشر: | 2006 |
| المجموعة: | Universidade Federal de São Paulo (UNIFESP): Repositório Institucional |
| مصطلحات موضوعية: | heat-labile toxin, enterotoxigenic Escherichia coli, labile toxin production/secretion, diarrheal disease |
| الوصف: | Production and release of heat-labile toxin (LT) by wild-type enterotoxigenic Escherichia coli (ETEC) strains, isolated from diarrheic and asymptomatic Brazilian children, was studied under in vitro and in vivo conditions. Based on a set of 26 genetically diverse LT+ enterotoxigenic E. coli strains, cell-bound LT concentrations varied from 49.8 to 2415 ng mL(-1). the amounts of toxin released in culture supernatants ranged from 0% to 50% of the total synthesized toxin. the amount of LT associated with secreted membrane vesicles represented < 5% of the total toxin detected in culture supernatants. ETEC strains secreting higher amounts of LT, but not those producing high intracellular levels of cell-bound toxin, elicited enhanced fluid accumulation in tied rabbit ileal loops, suggesting that the strain-specific differences in production and secretion of LT correlates with symptoms induced in vivo. However, no clear correlation was established between the ability to produce and secrete LT and the clinical symptoms of the infected individuals. the present results indicate that production and release of LT by wild-type human-derived ETEC strains are heterogeneous traits under both in vitro and in vivo growth conditions and may impact the clinical outcomes of infected individuals. ; Univ São Paulo, Dept Microbiol, Inst Ciencias Biomed, São Paulo, Brazil ; Universidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil ; Fdn Oswaldo Cruz, Escola Nacl Saude Publ, Rio de Janeiro, Brazil ; Univ São Paulo, Dept Patol, Fac Med Vet & Zootecn, São Paulo, Brazil ; Inst Butantan, Div Desenvolvimento Tecnol & Prod, São Paulo, Brazil ; Universidade Federal de São Paulo, Dept Microbiol Imunol & Parasitol, São Paulo, Brazil ; Web of Science |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | 123-131 |
| اللغة: | English |
| تدمد: | 0928-8244 |
| العلاقة: | Fems Immunology and Medical Microbiology; http://dx.doi.org/10.1111/j.1574-695X.2006.00134.xTest; Fems Immunology and Medical Microbiology. Oxford: Blackwell Publishing, v. 48, n. 1, p. 123-131, 2006.; http://repositorio.unifesp.br/handle/11600/29166Test; WOS:000240402700016 |
| DOI: | 10.1111/j.1574-695X.2006.00134.x |
| الإتاحة: | https://doi.org/10.1111/j.1574-695X.2006.00134.xTest http://repositorio.unifesp.br/handle/11600/29166Test |
| حقوق: | Acesso aberto |
| رقم الانضمام: | edsbas.B483B8D3 |
| قاعدة البيانات: | BASE |
| تدمد: | 09288244 |
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| DOI: | 10.1111/j.1574-695X.2006.00134.x |