In utero and lactational exposure to vinclozolin and genistein induces genomic changes in the rat mammary gland
| العنوان: | In utero and lactational exposure to vinclozolin and genistein induces genomic changes in the rat mammary gland |
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| المؤلفون: | Martine Perrot-Applanat, Sophie Vacher, Ivan Bièche, Marc Pocard, Aurore Toullec, H. El Sheikh Saad |
| المصدر: | Journal of Endocrinology. 216:245-263 |
| بيانات النشر: | Bioscientifica, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | medicine.medical_specialty, Offspring, Endocrinology, Diabetes and Metabolism, Mammary gland, Morphogenesis, Endocrine Disruptors, Biology, Polymerase Chain Reaction, chemistry.chemical_compound, Mammary Glands, Animal, Endocrinology, Pregnancy, Internal medicine, Claudin-1, medicine, Animals, Lactation, Vinclozolin, Oxazoles, Perinatal Exposure, Myoepithelial cell, Genistein, Immunohistochemistry, Fungicides, Industrial, Rats, medicine.anatomical_structure, chemistry, Prenatal Exposure Delayed Effects, Female, Mammary gland morphogenesis, Hormone |
| الوصف: | Exposure to low doses of environmental estrogens such as bisphenol A and genistein (G) alters mammary gland development. The effects of environmental anti-androgens, such as the fungicide vinclozolin (V), on mammary gland morphogenesis are unknown. We previously reported that perinatal exposure to G, V, and the GV combination causes histological changes in the mammary gland during the peripubertal period, suggesting alterations to the peripubertal hormone response. We now investigate whether perinatal exposure to these compounds alters the gene expression profiles of the developing glands to identify the dysregulated signaling pathways and the underlying mechanisms. G, V, or GV (1 mg/kg body weight per day) was added to diet of Wistar rats, from conception to weaning; female offspring mammary glands were collected at postnatal days (PNDs) 35 and 50. Genes displaying differential expression and belonging to different functional categories were validated by quantitative PCR and immunocytochemistry. At PND35, G had little effect; the slight changes noted were in genes related to morphogenesis. The changes following exposure to V concerned the functional categories associated with development (Cldn1,Krt17, andSprr1a), carbohydrate metabolism, and steroidogenesis. The GV mixture upregulated genes (Krt17,Pvalb, andTnni2) involved in muscle development, indicating effects on myoepithelial cells during mammary gland morphogenesis. Importantly, at PND50, cycling females exposed to GV showed an increase in the expression of genes (Csn2,Wap, andElf5) related to differentiation, consistent with the previously reported abnormal lobuloalveolar development previously described. Thus, perinatal exposure to GV alters the mammary gland hormone response differently at PND35 (puberty) and in animals with established cycles. |
| تدمد: | 1479-6805 0022-0795 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c9ee7d08046236e765ea32376c1d5a99Test https://doi.org/10.1530/joe-12-0395Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....c9ee7d08046236e765ea32376c1d5a99 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14796805 00220795 |
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