Substrate specificity of the lactate dehydrogenase isoenzyme C4 from human spermatozoa and a possible selective assay
| العنوان: | Substrate specificity of the lactate dehydrogenase isoenzyme C4 from human spermatozoa and a possible selective assay |
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| المؤلفون: | N M Gerez de Burgos, Carlos E. Coronel, Antonio Blanco, C Burgos |
| المصدر: | Reproduction. 55:101-106 |
| بيانات النشر: | Bioscientifica, 1979. |
| سنة النشر: | 1979 |
| مصطلحات موضوعية: | Male, Embryology, Electrophoresis, Starch Gel, Biology, Isozyme, Substrate Specificity, chemistry.chemical_compound, Endocrinology, Lactate dehydrogenase, Humans, Pyruvates, Caproates, Gel electrophoresis, chemistry.chemical_classification, L-Lactate Dehydrogenase, Myocardium, Obstetrics and Gynecology, Cell Biology, Spermatozoa, Sperm, Molecular biology, In vitro, Isoenzymes, Electrophoresis, Enzyme, Liver, Reproductive Medicine, chemistry, Biochemistry, Substrate specificity |
| الوصف: | The activity of lactate dehydrogenase (EC 1.1.1.27) in normal human sperm lysates and in human heart and liver homogenates was determined by using a variety of 2-oxoacids as substrates. Sperm preparations were active with pyruvate, 2-oxobutanoate, 2-oxopentanoate and 2-oxohexanoate, while heart and liver extracts utilized only pyruvate and 2-oxobutanoate. Selective staining after gel electrophoresis indicated that the fraction corresponding to lactate dehydrogenase C4, the sperm-specific isoenzyme, was responsible for the utilization of substrates with a linear chain of 3 to 6 carbon atoms. The use of 5 mM 2-oxohexanoate allowed the selective determination of isoenzyme C4 in preparations containing different lactate dehydrogenase molecular forms. |
| تدمد: | 1741-7899 1470-1626 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::9f65aa90631862f51d00c8db3d9eebd8Test https://doi.org/10.1530/jrf.0.0550101Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....9f65aa90631862f51d00c8db3d9eebd8 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17417899 14701626 |
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