Pro-inflammatory cytokines increase glucose, alanine and triacylglycerol utilization but inhibit insulin secretion in a clonal pancreatic β-cell line
| العنوان: | Pro-inflammatory cytokines increase glucose, alanine and triacylglycerol utilization but inhibit insulin secretion in a clonal pancreatic β-cell line |
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| المؤلفون: | Peter R. Flatt, Neville H. McClenaghan, Aoife Kiely, Philip Newsholme |
| المصدر: | Journal of Endocrinology. 195:113-123 |
| بيانات النشر: | Bioscientifica, 2007. |
| سنة النشر: | 2007 |
| مصطلحات موضوعية: | medicine.medical_specialty, Cytochalasin D, Necrosis, Glutamine, Endocrinology, Diabetes and Metabolism, medicine.medical_treatment, Interleukin-1beta, Glutamic Acid, Carbohydrate metabolism, Biology, Cell Line, Proinflammatory cytokine, Interferon-gamma, chemistry.chemical_compound, Adenosine Triphosphate, Endocrinology, Insulin-Secreting Cells, Internal medicine, Insulin Secretion, medicine, Animals, Insulin, Lactic Acid, Triglycerides, Analysis of Variance, Alanine, L-Lactate Dehydrogenase, Tumor Necrosis Factor-alpha, Glutathione, Metabolism, Glucose, chemistry, Apoptosis, Cytokines, medicine.symptom, Beta cell |
| الوصف: | We have investigated the effects of prolonged exposure (24 h) to pro-inflammatory cytokines on beta-cell metabolism and insulin secretion using clonal BRIN-BD11 beta cells. Addition of IL-1beta, tumour necrosis factor-alpha and IFN-gamma (at concentrations that did not induce apoptosis) inhibited chronic (24 h) and acute stimulated levels of insulin release (by 59 and 93% respectively), increased cellular glucose and alanine consumption, and also elevated lactate and glutamate release. However, ATP levels and cellular triacylglycerol were decreased while glutathione was increased. We conclude that sub-lethal concentrations of pro-inflammatory cytokines appear to shift beta-cell metabolism away from a key role in energy generation and stimulus-secretion coupling and towards a catabolic state which may be related to cell defence. |
| تدمد: | 1479-6805 0022-0795 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::777f57b851d37a934b86ac86d9abd874Test https://doi.org/10.1677/joe-07-0306Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....777f57b851d37a934b86ac86d9abd874 |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14796805 00220795 |
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