التفاصيل البيبلوغرافية
| العنوان: |
Unravelling the RNA-Binding Properties of SAFB Proteins in Breast Cancer Cells |
| المؤلفون: |
Elaine Hong, Andrew Best, Hannah Gautrey, Jas Chin, Anshuli Razdan, Tomaz Curk, David J. Elliott, Alison J. Tyson-Capper |
| بيانات النشر: |
BioMed Research International |
| سنة النشر: |
2015 |
| المجموعة: |
Hindawi Publishing Corporation |
| مصطلحات موضوعية: |
Molecular Biology |
| الوصف: |
Scaffold attachment factor B1 (SAFB1) and SAFB2 proteins are oestrogen (ER) corepressors that bind to and modulate ER activity through chromatin remodelling or interaction with the basal transcription machinery. SAFB proteins also have an internal RNA-recognition motif but little is known about the RNA-binding properties of SAFB1 or SAFB2. We utilised crosslinking and immunoprecipitation (iCLIP) coupled with high-throughput sequencing to enable a transcriptome-wide mapping of SAFB1 protein-RNA interactions in breast cancer MCF-7 cells. Analysis of crosslinking frequency mapped to transcript regions revealed that SAFB1 binds to coding and noncoding RNAs (ncRNAs). The highest proportion of SAFB1 crosslink sites mapped to ncRNAs, followed by intergenic regions, open reading frames (ORFs), introns, and 3′ or 5′ untranslated regions (UTR). Furthermore, we reveal that SAFB1 binds directly to RNA and its binding is particularly enriched at purine-rich sequences not dissimilar to the RNA-binding motifs for SR proteins. Using RNAi, we also show, for the first time, that single depletion of either SAFB1 or SAFB2 leads to an increase in expression of the other SAFB protein in both MCF-7 and MDA-MD231 breast cancer cells. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| العلاقة: |
https://doi.org/10.1155/2015/395816Test |
| DOI: |
10.1155/2015/395816 |
| الإتاحة: |
https://doi.org/10.1155/2015/395816Test |
| حقوق: |
Copyright © 2015 Elaine Hong et al. |
| رقم الانضمام: |
edsbas.493277E4 |
| قاعدة البيانات: |
BASE |
