التفاصيل البيبلوغرافية
| العنوان: |
Discovery of genes implicated in whirling disease infection and resistance in rainbow trout using genome-wide expression profiling |
| المؤلفون: |
Baerwald, Melinda R, Welsh, Amy B, Hedrick, Ronald P, May, Bernie |
| بيانات النشر: |
BioMed Central Ltd. |
| سنة النشر: |
2008 |
| المجموعة: |
BioMed Central |
| الوصف: |
Background Whirling disease, caused by the pathogen Myxobolus cerebralis , afflicts several salmonid species. Rainbow trout are particularly susceptible and may suffer high mortality rates. The disease is persistent and spreading in hatcheries and natural waters of several countries, including the U.S.A., and the economic losses attributed to whirling disease are substantial. In this study, genome-wide expression profiling using cDNA microarrays was conducted for resistant Hofer and susceptible Trout Lodge rainbow trout strains following pathogen exposure with the primary objective of identifying specific genes implicated in whirling disease resistance. Results Several genes were significantly up-regulated in skin following pathogen exposure for both the resistant and susceptible rainbow trout strains. For both strains, response to infection appears to be linked with the interferon system. Expression profiles for three genes identified with microarrays were confirmed with qRT-PCR. Ubiquitin-like protein 1 was up-regulated over 100 fold and interferon regulating factor 1 was up-regulated over 15 fold following pathogen exposure for both strains. Expression of metallothionein B , which has known roles in inflammation and immune response, was up-regulated over 5 fold in the resistant Hofer strain but was unchanged in the susceptible Trout Lodge strain following pathogen exposure. Conclusion The present study has provided an initial view into the genetic basis underlying immune response and resistance of rainbow trout to the whirling disease parasite. The identified genes have allowed us to gain insight into the molecular mechanisms implicated in salmonid immune response and resistance to whirling disease infection. |
| نوع الوثيقة: |
article in journal/newspaper |
| اللغة: |
English |
| العلاقة: |
http://www.biomedcentral.com/1471-2164/9/37Test |
| الإتاحة: |
http://www.biomedcentral.com/1471-2164/9/37Test |
| حقوق: |
Copyright 2008 Baerwald et al; licensee BioMed Central Ltd. |
| رقم الانضمام: |
edsbas.86573ADA |
| قاعدة البيانات: |
BASE |
