التفاصيل البيبلوغرافية
| العنوان: |
Anti-tumor activity of patient-derived NK cells after cell-based immunotherapy – a case report |
| المؤلفون: |
Milani, Valeria, Stangl, Stefan, Issels, Rolf, Gehrmann, Mathias, Wagner, Beate, Hube, Kathrin, Mayr, Doris, Hiddemann, Wolfgang, Molls, Michael, Multhoff, Gabriele |
| بيانات النشر: |
BioMed Central Ltd. |
| سنة النشر: |
2009 |
| المجموعة: |
BioMed Central |
| الوصف: |
Background Membrane-bound heat shock protein 70 (Hsp70) serves as a tumor-specific recognition structure for Hsp70-peptide (TKD) plus IL-2 activated NK cells. A phase I clinical trial has shown that repeated re-infusions of ex vivo TKD/IL-2-activated, autologous leukapheresis product is safe. This study investigated the maintenance of the cytolytic activity of NK cells against K562 cells and autologous tumor after 6 plus 3 infusions of TKD/IL-2-activated effector cells. Methods A stable tumor cell line was generated from the resected anastomotic relapse of a patient with colon carcinoma (pT3, N2, M0, G2). Two months after surgery, the patient received the first monthly i.v. infusion of his ex vivo TKD/IL-2-activated peripheral blood mononuclear cells (PBMNC). After 6 infusions and a pause of 3 months, the patient received another 3 cell infusions. The phenotypic characteristics and activation status of tumor and effector cells were determined immediately before and at times after each infusion. Results The NK cell ligands Hsp70, MICA/B, and ULBP-1,2,3 were expressed on the patient's anastomotic relapse. An increased density of activatory NK cell receptors following ex vivo stimulation correlated with an enhanced anti-tumoricidal activity. After 4 re-infusion cycles, the intrinsic cytolytic activity of non-stimulated PBMNC was significantly elevated and this heightened responsiveness persisted for up to 3 months after the last infusion. Another 2 re-stimulations with TKD/IL-2 restored the cytolytic activity after the therapeutic pause. Conclusion In a patient with colon carcinoma, repeated infusions of ex vivo TKD/IL-2-activated PBMNC initiate an intrinsic NK cell-mediated cytolytic activity against autologous tumor cells. |
| نوع الوثيقة: |
report |
| اللغة: |
English |
| العلاقة: |
http://www.translational-medicine.com/content/7/1/50Test |
| الإتاحة: |
http://www.translational-medicine.com/content/7/1/50Test |
| حقوق: |
Copyright 2009 Milani et al; licensee BioMed Central Ltd. |
| رقم الانضمام: |
edsbas.84E442D4 |
| قاعدة البيانات: |
BASE |
