التفاصيل البيبلوغرافية
| العنوان: |
Low density lipoprotein from patients with Type 2 diabetes increases expression of monocyte matrix metalloproteinase and ADAM metalloproteinase genes |
| المؤلفون: |
Worley, Joanna R, Hughes, David A, Dozio, Nicoletta, Gavrilovic, Jelena, Sampson, Mike J |
| بيانات النشر: |
BioMed Central Ltd. |
| سنة النشر: |
2007 |
| المجموعة: |
BioMed Central |
| الوصف: |
Aims Type 2 diabetes is characterised by increased plasma concentrations of pro-inflammatory cytokines [such as tumour necrosis factor – alpha; TNF-α] and soluble forms of adhesion molecules involved in leukocyte – endothelial interactions. These molecules are synthesised as transmembrane proteins and the plasma soluble forms are generated by ectodomain cleavage from the cell surface by members of the ADAM [ a d isintegrin a nd m etalloproteinase] proteinase family. We hypothesised that plasma low density lipoprotein [LDL] from subjects with Type 2 diabetes would influence in vitro monocytic ADAM and matrix metalloproteinase [MMP] gene expression differently compared to control LDL. Methods We examined relative mRNA expression by real time PCR in a monocytic cell line [THP-1] cultured for 4, 8 and 24 hrs with human plasma LDL derived from subjects with [n = 5] or without [n = 4] Type 2 diabetes. Gene expression for MMP-1 and 9, and ADAM – 8, 15, 17 and 28 was studied. Results Type 2 diabetes LDL significantly increased gene expression of MMP – 1 [p < 0.01] MMP – 9 [p < 0.001], and ADAM 17 [p < 0.05], – 28 [p < 0.01] and – 15 [p < 0.01] compared to control LDL. Type 2 diabetes LDL had disparate effects on inhibitors of MMP. Conclusion These data suggest that Type 2 diabetes LDL could lead to increased adhesion molecule and TNF alpha cell surface shedding, and vascular plaque instability, by promoting increased expression of ADAM and MMP genes. |
| نوع الوثيقة: |
other/unknown material |
| اللغة: |
English |
| العلاقة: |
http://www.cardiab.com/content/6/1/21Test |
| الإتاحة: |
http://www.cardiab.com/content/6/1/21Test |
| حقوق: |
Copyright 2007 Worley et al; licensee BioMed Central Ltd. |
| رقم الانضمام: |
edsbas.8DD4856E |
| قاعدة البيانات: |
BASE |
