H2A.Z landscapes and dual modifications in pluripotent and multipotent stem cells underlie complex genome regulatory functions
| العنوان: | H2A.Z landscapes and dual modifications in pluripotent and multipotent stem cells underlie complex genome regulatory functions |
|---|---|
| المؤلفون: | Haruhiko Koseki, Jacob D. Jaffe, Mitsuhiro Endoh, Manching Ku, Steven A. Carr, Esther Rheinbay, Richard Koche, Bradley E. Bernstein |
| المساهمون: | Harvard University--MIT Division of Health Sciences and Technology, Koche, Richard Patrick |
| المصدر: | BioMed Central Ltd BioMed Central Genome Biology |
| بيانات النشر: | BioMed Central Ltd, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Pluripotent Stem Cells, Transcriptional Activation, bivalent, Biology, Histones, ChIP-Seq, Mice, Neural Stem Cells, Nucleosome, Animals, Humans, Enhancer, Induced pluripotent stem cell, Promoter Regions, Genetic, Embryonic Stem Cells, Genetics, Genome, Research, Multipotent Stem Cells, H2A.Z, Promoter, Acetylation, Embryonic stem cell, Chromatin, Histone, Enhancer Elements, Genetic, mass spectrometry, ubiquitination, biology.protein, Protein Processing, Post-Translational, Bivalent chromatin |
| الوصف: | Background The histone variant H2A.Z has been implicated in nucleosome exchange, transcriptional activation and Polycomb repression. However, the relationships among these seemingly disparate functions remain obscure. Results We mapped H2A.Z genome-wide in mammalian ES cells and neural progenitors. H2A.Z is deposited promiscuously at promoters and enhancers, and correlates strongly with H3K4 methylation. Accordingly, H2A.Z is present at poised promoters with bivalent chromatin and at active promoters with H3K4 methylation, but is absent from stably repressed promoters that are specifically enriched for H3K27 trimethylation. We also characterized post-translational modification states of H2A.Z, including a novel species dually-modified by ubiquitination and acetylation that is enriched at bivalent chromatin. Conclusions Our findings associate H2A.Z with functionally distinct genomic elements, and suggest that post-translational modifications may reconcile its contrasting locations and roles. |
| وصف الملف: | application/pdf |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::72f160380c48988615503afba37d4408Test http://hdl.handle.net/1721.1/73623Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....72f160380c48988615503afba37d4408 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |