دورية أكاديمية
Chick Hairy1 protein interacts with Sap18, a component of the Sin3/HDAC transcriptional repressor complex
| العنوان: | Chick Hairy1 protein interacts with Sap18, a component of the Sin3/HDAC transcriptional repressor complex |
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| المؤلفون: | Sheeba, Caroline J., Palmeirim, I., Andrade, Raquel P. |
| بيانات النشر: | BioMed Central (BMC) |
| سنة النشر: | 2007 |
| المجموعة: | Universidade of Minho: RepositóriUM |
| مصطلحات موضوعية: | Science & Technology |
| الوصف: | Background: The vertebrate adult axial skeleton, trunk and limb skeletal muscles and dermis of the back all arise from early embryonic structures called somites. Somites are symmetrically positioned flanking the embryo axial structures (neural tube and notochord) and are periodically formed in a anterior-posterior direction from the presomitic mesoderm. The time required to form a somite pair is constant and species-specific. This extraordinary periodicity is proposed to depend on an underlying somitogenesis molecular clock, firstly evidenced by the cyclic expression of the chick hairy1 gene in the unsegmented presomitic mesoderm with a 90 min periodicity, corresponding to the time required to form a somite pair in the chick embryo. The number of hairy1 oscillations at any given moment is proposed to provide the cell with both temporal and positional information along the embryo's anterior-posterior axis. Nevertheless, how this is accomplished and what biological processes are involved is still unknown. Aiming at understanding the molecular events triggered by the somitogenesis clock Hairy1 protein, we have employed the yeast two-hybrid system to identify Hairy1 interaction partners. Results: Sap18, an adaptor molecule of the Sin3/HDAC transcriptional repressor complex, was found to interact with the C-terminal portion of the Hairy1 protein in a yeast two-hybrid assay and the Hairy1/Sap18 interaction was independently confirmed by co-immunoprecipitation experiments. We have characterized the expression patterns of both sap18 and sin3a genes during chick embryo development, using in situ hybridization experiments. We found that both sap18 and sin3a expression patterns co-localize in vivo with hairy1 expression domains in chick rostral presomitic mesoderm and caudal region of somites. Conclusion: Hairy1 belongs to the hairy-enhancer-of-split family of transcriptional repressor proteins. Our results indicate that during chick somitogenesis Hairy1 may mediate gene transcriptional repression by recruiting the ... |
| نوع الوثيقة: | article in journal/newspaper |
| وصف الملف: | application/pdf |
| اللغة: | English |
| تدمد: | 1471-213X |
| العلاقة: | info:eu-repo/grantAgreement/FCT/POCI/POCI%2FBIA-BCM%2F62688%2F2004/PT; info:eu-repo/grantAgreement/FCT/PIDDAC/SFRH%2FBPD%2F9432%2F2002/PT; http://www.biomedcentral.comTest/; Sheeba, C. J., Palmeirim, I., & Andrade, R. P. (2007, July 10). Chick Hairy1 protein interacts with Sap18, a component of the Sin3/HDAC transcriptional repressor complex. BMC Developmental Biology. Springer Science and Business Media LLC. http://doi.org/10.1186/1471-213x-7-83Test; https://hdl.handle.net/1822/8703Test |
| DOI: | 10.1186/1471-213X-7-83 |
| الإتاحة: | https://doi.org/10.1186/1471-213X-7-83Test https://hdl.handle.net/1822/8703Test |
| حقوق: | info:eu-repo/semantics/openAccess |
| رقم الانضمام: | edsbas.12B2DB9B |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 1471213X |
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| DOI: | 10.1186/1471-213X-7-83 |