The cell morphogenesis ANGUSTIFOLIA (AN) gene, a plant homolog of CtBP/BARS, is involved in abiotic and biotic stress response in higher plants
| العنوان: | The cell morphogenesis ANGUSTIFOLIA (AN) gene, a plant homolog of CtBP/BARS, is involved in abiotic and biotic stress response in higher plants |
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| المؤلفون: | Nicholas P. Johnson, José Carlos Jiménez-López, Oteri M Oghoghomeh, Sarah Smith, Emma W. Gachomo, Lamine Baba-Moussa, Simeon O. Kotchoni, Anthony B Cooksey |
| المصدر: | Digital.CSIC. Repositorio Institucional del CSIC instname BMC Plant Biology |
| بيانات النشر: | BioMed Central, 2013. |
| سنة النشر: | 2013 |
| مصطلحات موضوعية: | 0106 biological sciences, Arabidopsis thaliana, Mutant, Morphogenesis, Arabidopsis, Pseudomonas syringae, Angustifolia, Plant Science, Biology, Cell morphology, 01 natural sciences, 03 medical and health sciences, Biotic stress, Gene Expression Regulation, Plant, Stress, Physiological, Botany, Gene, 030304 developmental biology, Plant Diseases, 0303 health sciences, Abiotic stress, Cell morphogenesis, Arabidopsis Proteins, Hydrogen Peroxide, biology.organism_classification, Cell biology, Droughts, Repressor Proteins, Oxidative Stress, T-DNA knockout mutant, 010606 plant biology & botany, Research Article |
| الوصف: | Background ANGUSTIFOLIA (AN), one of the CtBP family proteins, plays a major role in microtubule-dependent cell morphogenesis. Microarray analysis of mammalian AN homologs suggests that AN might function as a transcriptional activator and regulator of a wide range of genes. Genetic characterization of AN mutants suggests that AN might be involved in multiple biological processes beyond cell morphology regulation. Results Using a reverse genetic approach, we provide in this paper the genetic, biochemical, and physiological evidence for ANGUSTIFOLIA’s role in other new biological functions such as abiotic and biotic stress response in higher plants. The T-DNA knockout an-t1 mutant exhibits not only all the phenotypes of previously described angustifolia null mutants, but also copes better than wild type under dehydration and pathogen attack. The stress tolerance is accompanied by a steady-state modulation of cellular H2O2 content, malondialdehyde (MDA) derived from cellular lipid peroxidation, and over-expression of stress responsive genes. Our results indicate that ANGUSTIFOLIA functions beyond cell morphology control through direct or indirect functional protein interaction networks mediating other biological processes such as drought and pathogen attacks. Conclusions Our results indicate that the ANGUSTIFOLIA gene participates in several biochemical pathways controlling cell morphogenesis, abiotic, and biotic stress responses in higher plants. Our results suggest that the in vivo function of plant ANGUSTIFOLIA has been overlooked and it needs to be further studied beyond microtubule-dependent cell morphogenesis. This work was supported by the Rutgers-University start-up funds and the RU-FAIR Mini-Grant # 430074 to SOK. |
| DOI: | 10.1186/1471-2229-13-79 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::56a14bf5b65962b98fbc2e33ec8bfd06Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....56a14bf5b65962b98fbc2e33ec8bfd06 |
| قاعدة البيانات: | OpenAIRE |
| DOI: | 10.1186/1471-2229-13-79 |
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