ABCC transporters mediate insect resistance to multiple Bt toxins revealed by bulk segregant analysis
| العنوان: | ABCC transporters mediate insect resistance to multiple Bt toxins revealed by bulk segregant analysis |
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| المؤلفون: | Rosa M. González-Martínez, Yonggyun Kim, Salvador Herrero, José Blanca, Pello Ziarsolo, Joaquín Cañizares, Juan Ferré, Youngjin Park, Maissa Chakroun, Gloria Navarro-Cerrillo |
| المصدر: | BMC Biology RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname |
| بيانات النشر: | BioMed Central, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Male, Physiology, Genes, Insect, Plant Science, Bt resistance, Insecticide Resistance, Hemolysin Proteins, Structural Biology, Bacillus thuringiensis, Chromosome Segregation, Phylogeny, Genetics, biology, Agricultural and Biological Sciences(all), Larva, Female, General Agricultural and Biological Sciences, Biotechnology, Research Article, Molecular Sequence Data, Spodoptera, ABCC2 transporter, Polymorphism, Single Nucleotide, General Biochemistry, Genetics and Molecular Biology, Bacterial Proteins, Exigua, Animals, Amino Acid Sequence, Gene, Ecology, Evolution, Behavior and Systematics, Crosses, Genetic, Bombyx, Bacillus thuringiensis Toxins, Biochemistry, Genetics and Molecular Biology(all), Gene Expression Profiling, fungi, Wild type, Cell Biology, Sequence Analysis, DNA, biology.organism_classification, Molecular biology, Endotoxins, Kinetics, GENETICA, Cry1Ac, Membrane protein, ATP-Binding Cassette Transporters, Cry toxins, Developmental Biology |
| الوصف: | [EN] Background: Relatively recent evidence indicates that ABCC2 transporters play a main role in the mode of action of Bacillus thuringiensis (Bt) Cry1A-type proteins. Mapping of major Cry1A resistance genes has linked resistance to the ABCC2 locus in Heliothis virescens, Plutella xylostella, Trichoplusia ni and Bombyx mori, and mutations in this gene have been found in three of these Bt-resistant strains. Results: We have used a colony of Spodoptera exigua (Xen-R) highly resistant to a Bt commercial bioinsecticide to identify regions in the S. exigua genome containing loci for major resistance genes by using bulk segregant analysis (BSA). Results reveal a region containing three genes from the ABCC family (ABBC1, ABBC2 and ABBC3) and a mutation in one of them (ABBC2) as responsible for the resistance of S. exigua to the Bt commercial product and to its key Spodoptera-active ingredients, Cry1Ca. In contrast to all previously described mutations in ABCC2 genes that directly or indirectly affect the extracellular domains of the membrane protein, the ABCC2 mutation found in S. exigua affects an intracellular domain involved in ATP binding. Functional analyses of ABBC2 and ABBC3 support the role of both proteins in the mode of action of Bt toxins in S. exigua. Partial silencing of these genes with dsRNA decreased the susceptibility of wild type larvae to both Cry1Ac and Cry1Ca. In addition, reduction of ABBC2 and ABBC3 expression negatively affected some fitness components and induced up-regulation of arylphorin and repat5, genes that respond to Bt intoxication and that are found constitutively up-regulated in the Xen-R strain. Conclusions: The current results show the involvement of different members of the ABCC family in the mode of action of B. thuringiensis proteins and expand the role of the ABCC2 transporter in B. thuringiensis resistance beyond the Cry1A family of proteins to include Cry1Ca. We want to thank C. S. Hern ndez-Rodr guez for her comments on binding assays, Ismael Mingarro for his help in determination of ABCC domains and William Moar (Auburn University, Auburn, AL) for his comments on the manuscript and generating the Xen-R colony. This research was partially supported by IPET (Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries), Ministry of Agriculture, Food and Rural Affairs to YK. Research at the University of Valencia was supported by Generalitat Valenciana (prometeo/2011/044) and Ministry of Science and Innovation (AGL2011-30352-C02-02 and AGL2012-39946-C02-01). |
| وصف الملف: | application/pdf |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::f3d3179b7afca7f4defc31d4a036e199Test https://doi.org/10.1186/1741-7007-12-46Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....f3d3179b7afca7f4defc31d4a036e199 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |