دورية أكاديمية
Pulsed-laser irradiation of multifunctional gold nanoshells to overcome trastuzumab resistance in HER2-overexpressing breast cancer
| العنوان: | Pulsed-laser irradiation of multifunctional gold nanoshells to overcome trastuzumab resistance in HER2-overexpressing breast cancer |
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| المؤلفون: | Nunes, Toni, Pons, Thomas, Hou, Xue, van Do, Khanh, Caron, Benoît, Rigal, Marthe, Di Benedetto, Melanie, Palpant, Bruno, Leboeuf, Christophe, Janin, Anne, Bousquet, Guilhem |
| المساهمون: | Marqueurs cardiovasculaires en situation de stress (MASCOT (UMR_S_942 / U942)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal Paris, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Université Sorbonne Paris Nord, Laboratoire de Physique et d'Etude des Matériaux (UMR 8213) (LPEM), Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris Sciences et Lettres (PSL)-Université Paris Sciences et Lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Photonique Quantique et Moléculaire (LPQM), École normale supérieure - Cachan (ENS Cachan)-CentraleSupélec-Centre National de la Recherche Scientifique (CNRS), Institut des Sciences de la Terre de Paris (iSTeP), Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Service de Pharmacie Hôpital Avicenne - APHP, Hôpital Avicenne AP-HP, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Laboratoire de pathologie AP-HP Hôpital Saint-Louis, Hopital Saint-Louis AP-HP (AP-HP), Service d'Oncologie Médicale AP-HP Hôpital Avicenne, Université Paris 13 (UP13)-Hôpital Avicenne AP-HP, This work received financial support from Labex LaSIPS (ANR-10-LABX0040– LaSIPS) managed by the French National Research Agency under the “Investissements d’avenir” program (n°ANR-11-IDEX-0003–02), from the “Plan Cancer” managed by the French ITMO Cancer (n°17CP077–00, project HEPPROS) and from the Institut d’Alembert in Ecole Normale Supérieure Paris-Saclay (FR CNRS 3242) (GESPER project)., ANR-11-IDEX-0003,IPS,Idex Paris-Saclay(2011) |
| المصدر: | ISSN: 1756-9966 ; Journal of experimental & clinical cancer research ; https://inserm.hal.science/inserm-02528127Test ; Journal of experimental & clinical cancer research, 2019, 38 (1), pp.306. ⟨10.1186/s13046-019-1305-x⟩. |
| بيانات النشر: | HAL CCSD BioMed Central |
| سنة النشر: | 2019 |
| مصطلحات موضوعية: | Femtosecond laser, Functionalized gold nanoparticles, HER2-overexpressing breast cancer, Photothermal therapy, Resistance reversion, Trastuzumab resistance, [SDV.CAN]Life Sciences [q-bio]/Cancer, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, [SDV.BIO]Life Sciences [q-bio]/Biotechnology, [SDV.TOX]Life Sciences [q-bio]/Toxicology |
| الوصف: | International audience ; BACKGROUND:HER2-overexpressing metastatic breast cancers are challenging practice in oncology when they become resistant to anti-HER2 therapies such as trastuzumab. In these clinical situations, HER2-overexpression persists in metastatic localizations, and can thus be used for active targeting using innovative therapeutic approaches. Functionalized gold nanoparticles with anti-HER2 antibody can be stimulated by near-infrared light to induce hyperthermia.METHODS:Here, hybrid anti-HER2 gold nanoshells were engineered for photothermal therapy to overcome trastuzumab resistance in HER2-overexpressing breast cancer xenografts.RESULTS:When gold nanoshells were administered in HER2-tumor xenografts, no toxicity was observed. A detailed pharmacokinetic study showed a time-dependent accumulation of gold nanoshells within the tumors, significantly greater with functionalized gold nanoshells at 72 h. This enabled us to optimize the treatment protocol and irradiate the mice when the anti-HER2 gold nanoshells had accumulated most in the tumors. After weekly injections of anti-HER2 gold nanoshells, and repeated irradiations with a femtosecond-pulsed laser over four weeks, tumor growth was significantly inhibited. Detailed tissue microscopic analyses showed that the tumor growth inhibition was due to an anti-angiogenic effect, coherent with a preferential distribution of the nanoshells in tumor microvessels. We also showed a direct tumor cell effect with apoptosis and inhibition of proliferation, coherent with an immune-mediated targeting of tumor cells by anti-HER2 nanoshells.CONCLUSION:This preclinical study thus supports the use of anti-HER2 gold nanoshells and photothermal therapy to overcome trastuzumab resistance in HER2-overexpressing breast cancer. |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| العلاقة: | info:eu-repo/semantics/altIdentifier/pmid/31299997; inserm-02528127; https://inserm.hal.science/inserm-02528127Test; https://inserm.hal.science/inserm-02528127/documentTest; https://inserm.hal.science/inserm-02528127/file/s13046-019-1305-x.pdfTest; PUBMED: 31299997; PUBMEDCENTRAL: PMC6626398 |
| DOI: | 10.1186/s13046-019-1305-x |
| الإتاحة: | https://doi.org/10.1186/s13046-019-1305-xTest https://inserm.hal.science/inserm-02528127Test https://inserm.hal.science/inserm-02528127/documentTest https://inserm.hal.science/inserm-02528127/file/s13046-019-1305-x.pdfTest |
| حقوق: | info:eu-repo/semantics/OpenAccess |
| رقم الانضمام: | edsbas.647FB3CE |
| قاعدة البيانات: | BASE |
| DOI: | 10.1186/s13046-019-1305-x |
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