Performance of Mycobacterium Growth Indicator Tube BACTEC 960 with Lowenstein–Jensen method for diagnosis of Mycobacterium tuberculosis at Ethiopian National Tuberculosis Reference Laboratory, Addis Ababa, Ethiopia
| العنوان: | Performance of Mycobacterium Growth Indicator Tube BACTEC 960 with Lowenstein–Jensen method for diagnosis of Mycobacterium tuberculosis at Ethiopian National Tuberculosis Reference Laboratory, Addis Ababa, Ethiopia |
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| المؤلفون: | Zekarias Dagne, Zelalem Yaregal, Abebaw Kebede, Abyot Meaza, Kebebe Gudena, Getu Diriba, Kassu Desta, Shewki Moga, Jibril Dilebo, Mulu Hassen, Muluwork Getahun, Mengistu Tadesse |
| المصدر: | BMC Research Notes BMC Research Notes, Vol 10, Iss 1, Pp 1-6 (2017) |
| بيانات النشر: | BioMed Central, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Veterinary medicine, Mycobacterium complex, lcsh:Medicine, 0302 clinical medicine, Technical Note, Mycobacteria growth indicator tube, 030212 general & internal medicine, Child, lcsh:QH301-705.5, Aged, 80 and over, biology, General Medicine, Middle Aged, Child, Preschool, Bronchoalveolar Lavage Fluid, Adult, medicine.medical_specialty, Tuberculosis, Diagnostic methods, Adolescent, 030106 microbiology, Reference laboratory, Lowenstein–Jensen, Sensitivity and Specificity, General Biochemistry, Genetics and Molecular Biology, Specimen Handling, Mycobacterium tuberculosis, 03 medical and health sciences, Young Adult, Recovery rate, medicine, Humans, lcsh:Science (General), Aged, Bacteriological Techniques, business.industry, Mycobacteria Growth Indicator Tube, lcsh:R, Sputum, Reproducibility of Results, medicine.disease, biology.organism_classification, Surgery, Cross-Sectional Studies, lcsh:Biology (General), Ethiopia, business, lcsh:Q1-390, Mycobacterium |
| الوصف: | Background Bacteriological confirmed active case detection remains the corner stone for diagnosing tuberculosis. Non-radiometric liquid culture system Mycobacterium Growth Indicator Tube with automated interface had been recommended by expert groups in addition to conventional solid culture media such as Lowenstein–Jensen. However in high burden resource limited countries advanced non-radiometric based tuberculosis diagnostic methods such as MGIT 960 is limited. Therefore we have evaluated the performance of MGIT 960 system compared to LJ for recovery of Mycobacterium complex (MTBC) from clinical specimens. Methods A cross sectional study was conducted from a total of 908 samples between January 1st, 2013 to December 31st, 2014. Clinical specimens were processed following standard procedures and the final suspension was inoculated to MGIT tubes and LJ slant. Identification and confirmation of MTBC was done by ZN staining and SD Bioline test. Data was analyzed by SPSS version 20. The sensitivity, specificity, recovery rate and the average turnaround time to recover the organism was computed. Results From a total of 908 clinical specimens processed using both LJ and BACTEC MGIT liquid culture methods the recovery rate for LJ and MGIT, for smear positive samples was 66.7% (74/111) and 87.4% (97/ 111) respectively while for smear negative samples was 13.4% (108/797) and 17.4% (139/797) for LJ and MGIT methods respectively. The overall recovery rate for MGIT is significantly higher than LJ methods [26% (236/908; vs. 20%, 182/908, P = 0.002)]. The average turnaround time for smear positive samples was 16 and 31 days for MGIT and LJ respectively. Turnaround time for smear negative samples was 20 and 36 days for MGIT and LJ respectively. The overall agreement between MGIT and LJ was fairly good with Kappa value of 0.59 (P |
| اللغة: | English |
| تدمد: | 1756-0500 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ba452f8e16955139f1a08c02c5c20ddaTest http://europepmc.org/articles/PMC5424417Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ba452f8e16955139f1a08c02c5c20dda |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17560500 |
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