DREAM regulates BDNF-dependent spinal sensitization
| العنوان: | DREAM regulates BDNF-dependent spinal sensitization |
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| المؤلفون: | Jorge R. Barrio, J.A. Lopez-Garcia, Jose R. Naranjo, Tomaso Benedet, Begoña Torres, Ivan Rivera-Arconada, Britt Mellström, Agnieszka Krzyzanowska, Carlos Avendaño, Carolina Roza |
| المصدر: | Molecular Pain Molecular Pain, Vol 6, Iss 1, p 95 (2010) |
| بيانات النشر: | BioMed Central, 2010. |
| سنة النشر: | 2010 |
| مصطلحات موضوعية: | Genetically modified mouse, medicine.medical_specialty, Pain, Mice, Transgenic, Dynorphin, Cellular and Molecular Neuroscience, Mice, Neurotrophic factors, Internal medicine, lcsh:Pathology, medicine, Animals, Sensitization, Brain-derived neurotrophic factor, Inflammation, Nerve Fibers, Unmyelinated, business.industry, Research, Brain-Derived Neurotrophic Factor, Wild type, Kv Channel-Interacting Proteins, Spinal cord, Rats, Repressor Proteins, medicine.anatomical_structure, Endocrinology, Anesthesiology and Pain Medicine, Gene Expression Regulation, Spinal Cord, Hyperalgesia, Molecular Medicine, Mutant Proteins, medicine.symptom, business, Neuroscience, psychological phenomena and processes, lcsh:RB1-214 |
| الوصف: | Background: The transcriptional repressor DREAM (downstream regulatory element antagonist modulator) controls the expression of prodynorphin and has been involved in the modulation of endogenous responses to pain. To investigate the role of DREAM in central mechanisms of pain sensitization, we used a line of transgenic mice (L1) overexpressing a Ca2+- and cAMP-insensitive DREAM mutant in spinal cord and dorsal root ganglia. Results: L1 DREAM transgenic mice showed reduced expression in the spinal cord of several genes related to pain, including prodynorphin and BDNF (brain-derived neurotrophic factor) and a state of basal hyperalgesia without change in A-type currents. Peripheral inflammation produced enhancement of spinal reflexes and increased expression of BDNF in wild type but not in DREAM transgenic mice. The enhancement of the spinal reflexes was reproduced in vitro by persistent electrical stimulation of C-fibers in wild type but not in transgenic mice. Exposure to exogenous BDNF produced a long-term enhancement of dorsal root-ventral root responses in transgenic mice. Conclusions: Our results indicate that endogenous BDNF is involved in spinal sensitization following inflammation and that blockade of BDNF induction in DREAM transgenic mice underlies the failure to develop spinal sensitization. |
| اللغة: | English |
| تدمد: | 1744-8069 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::e46ab161294cd88fae1d3833a5a3933aTest http://europepmc.org/articles/PMC3027194Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....e46ab161294cd88fae1d3833a5a3933a |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 17448069 |
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