Brilliant blue G, a P2X7 receptor antagonist, attenuates early phase of renal inflammation, interstitial fibrosis and is associated with renal cell proliferation in ureteral obstruction in rats
| العنوان: | Brilliant blue G, a P2X7 receptor antagonist, attenuates early phase of renal inflammation, interstitial fibrosis and is associated with renal cell proliferation in ureteral obstruction in rats |
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| المؤلفون: | Christina Maeda Takiya, Felipe M. Ornellas, Debora S. Ornellas, Marcelo M. Morales, José Monteiro Sad Pereira, Lucio R. Cardoso, André Luis Barreira, Luiz Carlos D. de Miranda, Alberto Schanaider, Maurilo Leite, Conrado Rodrigues Gomes, Robson Coutinho-Silva |
| المصدر: | BMC Nephrology BMC Nephrology, Vol 21, Iss 1, Pp 1-11 (2020) |
| بيانات النشر: | BioMed Central, 2020. |
| سنة النشر: | 2020 |
| مصطلحات موضوعية: | 0301 basic medicine, Nephrology, Male, Time Factors, Interleukin-1beta, Apoptosis, lcsh:RC870-923, urologic and male genital diseases, Kidney, 0302 clinical medicine, Fibrosis, Cell Movement, Rosaniline Dyes, Myofibroblasts, Nephritis, Renal inflammation, Purinergic receptor, female genital diseases and pregnancy complications, Up-Regulation, medicine.anatomical_structure, Kidney Tubules, P2X7 receptor, medicine.symptom, Research Article, Ureteral Obstruction, Collagen Type IV, medicine.medical_specialty, Purinergic P2X Receptor Antagonists, Antigens, Differentiation, Myelomonocytic, Inflammation, Collagen Type I, Transforming Growth Factor beta1, 03 medical and health sciences, Downregulation and upregulation, Antigens, CD, Internal medicine, medicine, Animals, RNA, Messenger, Rats, Wistar, HSP47 Heat-Shock Proteins, Cell Proliferation, business.industry, Cell growth, urogenital system, Macrophages, medicine.disease, lcsh:Diseases of the genitourinary system. Urology, Actins, Rats, 030104 developmental biology, Endocrinology, Collagen Type III, Unilateral ureteral obstruction, business, 030217 neurology & neurosurgery |
| الوصف: | Background Previous study showed that purinergic P2X7 receptors (P2X7R) reach the highest expression in the first week after unilateral ureteral obstruction (UUO) in mice, and are involved in the process of inflammation, apoptosis and fibrosis of renal tissue. We, herein, document the role of purinergic P2X7 receptors activation on the third day of UUO, as assessed by means of BBG as its selective inhibitor. Methods We investigated the effects of brilliant blue G (BBG), a P2X7R antagonist, in the third day of kidney tissue response to UUO in rats. For this purpose, male Wistar rats submitted to UUO or sham operated, received BBG or vehicle (V), comprising four groups: UUO-BBG, UUO-V, sham-BBG and sham-V. The kidneys were harvested on day 3 UUO and prepared for histology, immunohistochemistry (P2X7R, PCNA, CD-68, α-sma, TGF-β1, Heat-shock protein-47, TUNEL assay), quantitative real-time PCR (IL-1β, procollagens type I, III, and IV) for mRNA quantification. Results The group UUO-V presented an enhancement in tubular cell P2X7-R expression, increase influx of macrophages and myofibroblasts, HSP-47 and TGF- β1 expression. Also, upregulation of procollagen types I, III, and IV, and IL-1β mRNAs were seen. On the other hand, group UUO-BBG showed lower expression of procollagens and IL-1β mRNAs, as well as less immunoreactivity of HSP-47, TGF-β, macrophages, myofibroblasts, and tubular apoptosis. This group also presented increased epithelial cell proliferation. Conclusion BBG, a known highly selective inhibitor of P2X7R, attenuated renal inflammation, collagen synthesis, renal cell apoptosis, and enhanced renal cell proliferation in the early phase of rat model of UUO. |
| اللغة: | English |
| تدمد: | 1471-2369 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::05d8f45906aa6a378060b40c4ef53f4bTest http://europepmc.org/articles/PMC7260756Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....05d8f45906aa6a378060b40c4ef53f4b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 14712369 |
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