التفاصيل البيبلوغرافية
| العنوان: |
Acteoside protects podocyte against apoptosis through regulating AKT/GSK-3β signaling pathway in db/db mice. |
| المؤلفون: |
Li, Xiaoya1,2,3, Liu, Zhilong3,4, He, Zhixiu1,2,3, Wang, Xiaocheng5, Li, Rongshan1,2,3, Wang, Junwei3,4, Ma, Guiqiao3,4, Zhang, Peipei1,2,3, Ma, Chanjuan1,2,3 mcj5670206@163.com |
| المصدر: |
BMC Endocrine Disorders. 10/23/2023, Vol. 23 Issue 1, p1-11. 11p. |
| مصطلحات موضوعية: |
*KIDNEY physiology, *PROTEIN kinases, *BIOLOGICAL models, *ALBUMINS, *STAINS & staining (Microscopy), *ANIMAL experimentation, *IMMUNOHISTOCHEMISTRY, *WESTERN immunoblotting, *APOPTOSIS, *SIGNAL peptides, *DIABETES, *GLYCOSIDES, *CELLULAR signal transduction, *PREVENTIVE health services, *COMPARATIVE studies, *TRANSFERASES, *DESCRIPTIVE statistics, *RESEARCH funding, *EPITHELIAL cells, *DIABETIC nephropathies, *MICE, *LIPIDS, *ALBUMINURIA |
| مستخلص: |
Background: Podocyte apoptosis is one of the important pathological mechanisms of diabetic kidney disease (DKD). Acteoside (Act), a major active component of Rehmannia glutinosa leaves total glycoside, has a strong renoprotective action. Our study aims to demonstrate Act's renoprotective actions in db/db mice. Methods: We adopted C57BLKS/J db/db mice as DKD animal models. After 8 weeks of Act administration, the 24-hour urine albumin, renal function index, and blood lipid levels were quantified using matching kits. Renal pathology was evaluated by HE and PAS staining. The podocyte damage and apoptosis-related signaling pathway were observed by using immunohistochemistry, western blot, and TUNEL staining. Results: The albuminuria of db/db mice was reduced from 391 ug/24 h to 152 ug/24 h, and renal pathology changes were alleviated after Act administration. The western blot and immunohistochemistry showed that Act treatment upregulated the synaptopodin and podocin expression compared with db/db mice, while the TUNEL staining indicated podocyte apoptosis was inhibited. The B-cell lymphoma-2 (Bcl-2) level was upregulated in the Act group, but cleaved caspase-3 and Bcl-2 associated X protein (Bax) expression declined, while the protein kinase B/glycogen synthase kinase-3β (AKT/GSK-3β) signaling pathway was repressed. Conclusions: By inhibiting the AKT/GSK-3β signaling pathway, Act protected podocytes from apoptosis, decreasing the urine albumin of db/db mice and delaying the course of DKD. [ABSTRACT FROM AUTHOR] |
| قاعدة البيانات: |
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