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المصدر: Current Aging Science. 15:65-76
مصطلحات موضوعية: Pulmonary and Respiratory Medicine, business.industry, Parkinsonism, Antagonist, Adenosine A2A receptor, Rotenone, Pharmacology, medicine.disease, medicine.disease_cause, Adenosine, Dopamine agonist, chemistry.chemical_compound, chemistry, Pediatrics, Perinatology and Child Health, medicine, Curcumin, business, Oxidative stress, medicine.drug
الوصف: Background:Parkinsonism is a neurodegenerative disorder that affects elderly people worldwide.Methods:Curcumin, adenosine A2AR antagonist (ZM241385) and Sinemet® (L-dopa) were evaluated against Parkinson’s disease (PD) induced by rotenone in rats, and the findings were compared to our previous study on mice model.Results:Rats injected with rotenone showed severe alterations in adenosine A2A receptor gene expression, oxidative stress markers, inflammatory mediator, energetic indices, apoptotic marker and DNA fragmentation levels as compared to the control group. Treatments with curcumin, ZM241385, and Sinemet® restored all the selected parameters. The brain histopathological features of cerebellum regions confirmed our results. By comparing our results with the previous results on mice, we noticed that mice respond to rotenone toxicity and treatments more than rats with regards to behavioral observation, A2AR gene expression, neurotransmitter levels, inflammatory mediator and apoptotic markers, while rats showed higher response to treatments regarding oxidative stress and energetic indices.Conclusion:Curcumin succeeded in attenuating the severe effects of Parkinson’s disease in the rat model and can be considered as a potential dietary supplement. Adenosine A2AR antagonist has almost the same pattern of improvement as Sinemet® and may be considered as a promising therapy against PD. To compare the role of animal species in response to PD symptoms and treatments, our previous report on mice explored the response of mice to rotenone toxicity in comparison with rats, where rats have shown a higher response to treatments. Therefore, no animal model can perfectly recapitulate all the pathologies of PD.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::d335d9404a130563d741b218a72e1487Test
https://doi.org/10.2174/1874609814666210526115740Test -
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المؤلفون: Asmaa F. Aboul Naser, Amal M. El-Feky, Marwa M. Elbatanony, Manal A. Hamed, Azza A. Matloub, Nagy S. El-Rigal, Wagdy K. B. Khalil
المصدر: Current Bioactive Compounds. 17
مصطلحات موضوعية: chemistry.chemical_compound, Chemistry, Parkinsonism, medicine, Rotenone, General Pharmacology, Toxicology and Pharmaceutics, Pharmacology, medicine.disease, Citrus × sinensis
الوصف: Background: Parkinson’s disease (PD) is one of the most common neurodegenerative disorders spread worldwide in elderly people. Methods: The Citrus peels methanolic extract (100 mg/kg body weight) was evaluated as an antiparkinsonism agent in rats through estimation of oxidative stress markers, neurotransmitter levels, energetic indices, DNA fragmentation pattern, inflammatory mediators, adenosine A2A receptor gene expression and the histopathological analysis of the brain. In addition, its effect was compared with ZM241385; an adenosine A2A receptor antagonist, as well as the classical drug; (L-dopa). Results: The methanolic extract of C. sinensis peels constituted 17.59 ± 1.92 mg GAE/g and 4.88 ± 0.43 mg CE/g of total phenolic and flavonoid content, respectively. The polyphenolic composition was qualified and quantified using HPLC/DAD and UPLC/ESI-MS analysis. HPLC/DAD analysis led to identify 8 phenolic acids and 4 flavonoids. UPLC/MS analysis led to identify 20 polyphenolic compounds, including 9 polymethoxylated flavoniods, 7 flavonoidal glycosides and 4 phenolic derivatives. Nobiletin and tangeretin were found as abundant polymethoxylated flavones while, hesperidin and 1-caffeoyl-β-D-glucose were found as abundant glycosyl flavone and phenolic derivatives, respectively. Rotenone induced rats showed a significant decrease in neurotransmitter levels, energetic and antioxidant parameters, while a significant increase in total protein, inflammatory mediators, adenosine A2A receptor gene expression, DNA and lipid peroxidation levels was recorded. Treatments with plant extract, L-dopa and ZM241385 restored these selected parameters to variable extents with a more potent effect of ZM241385 than L-dopa. Rotenone induced rats were left free without treatment; not recorded a noticeable improvement level. Conclusion: Citrus sinensis peels was rich with bioactive valuable-added products. This may lead to the development of new nutraceutical and pharmaceutical agents as well as functional food products used as anti-oxidative, anti-inflammatory and anti-parkinsonian agent.
الوصول الحر: https://explore.openaire.eu/search/publication?articleId=doi_________::f0d83c4214a4a70d67d5246ddc10725bTest
https://doi.org/10.2174/1573407216999200918182514Test
