Chitinase 3-like 1 secreted by peritumoral macrophages in esophageal squamous cell carcinoma is a favorable prognostic factor for survival
| العنوان: | Chitinase 3-like 1 secreted by peritumoral macrophages in esophageal squamous cell carcinoma is a favorable prognostic factor for survival |
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| المؤلفون: | Xin Zheng, Mu-Sheng Zeng, Qian Zhong, Fan Hou, Chu Wei, Kai Lu Pan, Shan Xing, Yue Song Cao, Wan Li Liu, Tao Zeng |
| المصدر: | World Journal of Gastroenterology |
| بيانات النشر: | Baishideng Publishing Group Inc, 2017. |
| سنة النشر: | 2017 |
| مصطلحات موضوعية: | 0301 basic medicine, Male, Pathology, Esophageal Neoplasms, Macrophage, Kaplan-Meier Estimate, Esophageal squamous cell carcinoma, 0302 clinical medicine, Tumor Microenvironment, Chitinase-3-Like Protein 1, Aged, 80 and over, biology, Gastroenterology, Age Factors, General Medicine, Basic Study, Middle Aged, Prognosis, Immunohistochemistry, Chitinase 3-like 1, medicine.anatomical_structure, 030220 oncology & carcinogenesis, Carcinoma, Squamous Cell, Female, Adult, medicine.medical_specialty, Prognostic biomarker, Enzyme-Linked Immunosorbent Assay, 03 medical and health sciences, Esophagus, Cell Line, Tumor, medicine, Biomarkers, Tumor, Humans, Lymphocyte Count, Interleukin 6, Aged, Tumor microenvironment, business.industry, Interleukin-6, Macrophages, stomatognathic diseases, 030104 developmental biology, Chitinase, biology.protein, business |
| الوصف: | AIM To identify whether chitinase 3-like 1 (CHI3L1) serves as a suitable biomarker for the prognosis of esophageal squamous cell carcinoma (ESCC) and to analyze this protein’s cellular source. METHODS An ELISA was conducted to detect the concentration of CHI3L1 in the serum of 150 ESCC patients diagnosed between January 2001 and February 2005. The prognostic relevance of CHI3L1 was evaluated by a Kaplan-Meier and Cox regression analysis. The immunohistochemistry was reanalyzed, and fluorescent staining was utilized to explore the cellular origins of CHI3L1. We stimulated monocyte-derived macrophages (MDMs) with either IL-6 or the supernatant of the ESCC cell line Eca-109 and later investigated the level of CHI3L1 by qPCR and ELISA. RESULTS The level of serum CHI3L1 was higher in older patients (≥ 60) than in patients under the age of 60 (P = 0.001). The patients with higher levels of CHI3L1 had a significantly shorter overall survival, whereas the traditional markers, carcinoembryonic antigen and squamous cell carcinoma antigen, were less effective (P > 0.05). A multivariate Cox analysis (P = 0.001) indicated that CHI3L1 was an independent prognostic factor for ESCC patients. Peritumoral macrophages in ESCC exhibited high levels of CHI3L1. Interleukin-6 (IL-6) and the supernatant of Eca-109 containing IL-6 stimulated MDMs to secrete CHI3L1. The serum concentration of CHI3L1 in the ESCC patients showed a weak correlation with the laboratory inflammatory parameters neutrophil (NEU, P = 0.045), neutrophil/lymphocyte rate (NLR, P = 0.016), and C-reactive protein (CRP, P < 0.001). CONCLUSION Our study first established a connection between the pretreated CHI3L1 and patients with ESCC, and the serum CHI3L1 was primarily secreted by ESCC-surrounded macrophages. |
| اللغة: | English |
| تدمد: | 2219-2840 1007-9327 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3a876cfe28f54f62e891d00cc7fa727bTest http://europepmc.org/articles/PMC5703929Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3a876cfe28f54f62e891d00cc7fa727b |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 22192840 10079327 |
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