DNA template CdS nanowires (NW) have been successfully synthesized using single strand oligonucleatic sequences as templates. The synthesis was carried out by a simple chemical route where a mixture of cadmium sulphate and single stranded oligonucleotides (poly G and poly C) taken in a beaker for the formation of the DNA template and then add thiourea solution for formation of CdS-DNA NWs. The possible growth mechanism have been discussed in detail. Transmission electron microscopy (TEM) were used to characterize the DNA template CdSNWs. TEM results showed that the resulted CdSNWs were directly aligned on the DNA strand. CdSNWs grown in this method found to be larger than the length of DNA chosen for synthesis. The selected area diffraction pattern of transmission electron microscopy confirmed the zinc-blende CdS nanowires. The junction device, ITO/CdS-DNA/Auconfirmsthe ohmiccontact and enhancement in the forward bias current for CdS NW with respect to bare CdS nanoparticle establish the fact that DNA template CdS NW can be used for a nano-bioelectronic device. Although further investigations are required to study the mechanism of the charge transfer in DNA conjugated CdS NWs, these results can have important implications to the fabrication of more sophisticated and high multi-specificity biosensors and nano-bioelectronic devices.DNA template CdS nanowires (NW) have been successfully synthesized using single strand oligonucleatic sequences as templates. The synthesis was carried out by a simple chemical route where a mixture of cadmium sulphate and single stranded oligonucleotides (poly G and poly C) taken in a beaker for the formation of the DNA template and then add thiourea solution for formation of CdS-DNA NWs. The possible growth mechanism have been discussed in detail. Transmission electron microscopy (TEM) were used to characterize the DNA template CdSNWs. TEM results showed that the resulted CdSNWs were directly aligned on the DNA strand. CdSNWs grown in this method found to be larger than the length of DNA chosen for synthesis. The selected area diffraction pattern of transmission electron microscopy confirmed the zinc-blende CdS nanowires. The junction device, ITO/CdS-DNA/Auconfirmsthe ohmiccontact and enhancement in the forward bias current for CdS NW with respect to bare CdS nanoparticle establish the fact that DNA tem...