Scleral Proteome in Noninfectious Scleritis Unravels Upregulation of Filaggrin-2 and Signs of Neovascularization
| العنوان: | Scleral Proteome in Noninfectious Scleritis Unravels Upregulation of Filaggrin-2 and Signs of Neovascularization |
|---|---|
| المؤلفون: | Daphne P. C. Vergouwen, Josianne C. Ten Berge, Coskun Guzel, Thierry P. P. van den Bosch, Robert M. Verdijk, Aniki Rothova, Theo M. Luider, Marco W. J. Schreurs |
| المصدر: | Investigative Ophthalmology & Visual Science. 64(3):27-27 |
| بيانات النشر: | Association for Research in Vision and Ophthalmology Inc., 2023. |
| سنة النشر: | 2023 |
| مصطلحات موضوعية: | General Medicine |
| الوصف: | Purpose: Scleritis is a severe inflammatory ocular disorder with unknown pathogenesis. We investigated healthy sclera as well as sclera affected by noninfectious scleritis for differentially expressed proteins using a mass spectrometry approach. Methods: We collected scleral samples of enucleated eyes due to severe noninfectious scleritis (n = 3), and control scleral tissues (n = 5), all exenterated eyes for eyelid carcinomas (n = 4), or choroidal melanoma (n = 1) without scleral invasion. Samples were prepared for the nano liquid-chromatography mass spectrometer (LC-MS), data were analyzed using proteomics software (Scaffold), and is available via ProteomeXchange (identifier PXD038727). Samples were also stained for immuno-histopathological evaluation. Results: Mass spectrometry identified 629 proteins within the healthy and diseased scleral tissues, whereof collagen type XII, VI, and I were the most abundantly expressed protein. Collagen type II-XII was also present. Filaggrin-2, a protein that plays a crucial role in epidermal barrier function, was found upregulated in all scleritis cases. In addition, other epithelial associated proteins were upregulated (such as keratin 33b, 34, and 85, epiplakin, transglutaminase-3, galectin 7, and caspase-14) in scleritis. Further, upregulated proteins involved in regulation of the cytoskeleton (vinculin and myosin 9), and housekeeping proteins were found (elongation factor-2 and cytoplasmic dynein 1) in our study. Upregulation of filaggrin-2 and myosin-9 was confirmed with immunohistochemistry, the latter protein showing co-localization with the endothelial cell marker ETC-related gene (ERG), indicating neovascularization in scleral tissue affected by scleritis. Conclusions: We found upregulation of filaggrin-2 and signs of neovascularization in scleral tissue of patients with noninfectious scleritis. Further research, ideally including more scleritis cases, is needed to validate our findings. |
| اللغة: | English |
| تدمد: | 1552-5783 0146-0404 |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::ad677e0a9ce3393752574de2739fcfbdTest https://pure.eur.nl/en/publications/0facdd1e-982d-4b11-84e9-66694a60ce77Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....ad677e0a9ce3393752574de2739fcfbd |
| قاعدة البيانات: | OpenAIRE |
| تدمد: | 15525783 01460404 |
|---|