Effects of high-intensity intermittent training on carnitine palmitoyl transferase activity in the gastrocnemius muscle of rats
| العنوان: | Effects of high-intensity intermittent training on carnitine palmitoyl transferase activity in the gastrocnemius muscle of rats |
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| المؤلفون: | Marilia Seelaender, Waldecir Paula Lima, Robson Eder, Nelo Eidy Zanchi, Humberto Nicastro, Daniela Caetano Gonçalves, Fábio Santos Lira, Jean Marc Lavoie, Luiz Carlos Carnevali |
| المصدر: | Brazilian Journal of Medical and Biological Research, Volume: 45, Issue: 8, Pages: 777-783, Published: AUG 2012 Brazilian Journal of Medical and Biological Research, Vol 45, Iss 8, Pp 777-783 (2012) Brazilian Journal of Medical and Biological Research Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Brazilian Journal of Medical and Biological Research v.45 n.8 2012 Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
| بيانات النشر: | Associação Brasileira de Divulgação Científica, 2012. |
| سنة النشر: | 2012 |
| مصطلحات موضوعية: | Male, Physiology, Biochemistry, chemistry.chemical_compound, Random Allocation, Citrate synthase, General Pharmacology, Toxicology and Pharmaceutics, lcsh:QH301-705.5, computer.programming_language, Lipoprotein lipase, lcsh:R5-920, biology, sed, Reverse Transcriptase Polymerase Chain Reaction, General Neuroscience, General Medicine, Adaptation, Physiological, High-intensity intermittent training, Carnitine palmitoyl transferase, lcsh:Medicine (General), Oxidation-Reduction, medicine.drug, medicine.medical_specialty, Short Communication, Immunology, Biophysics, Real-Time Polymerase Chain Reaction, Gastrocnemius muscle, Internal medicine, Lactate dehydrogenase, Physical Conditioning, Animal, medicine, Animals, Carnitine O-palmitoyltransferase, Carnitine, Rats, Wistar, Muscle, Skeletal, Carnitine O-Palmitoyltransferase, Lipid metabolism, Cell Biology, HISTOLOGIA, Rats, Lipoprotein Lipase, Endocrinology, chemistry, lcsh:Biology (General), biology.protein, computer |
| الوصف: | We examined the capacity of high-intensity intermittent training (HI-IT) to facilitate the delivery of lipids to enzymes responsible for oxidation, a task performed by the carnitine palmitoyl transferase (CPT) system in the rat gastrocnemius muscle. Male adult Wistar rats (160-250 g) were randomly distributed into 3 groups: sedentary (Sed, N = 5), HI-IT (N = 10), and moderate-intensity continuous training (MI-CT, N = 10). The trained groups were exercised for 8 weeks with a 10% (HI-IT) and a 5% (MI-CT) overload. The HI-IT group presented 11.8% decreased weight gain compared to the Sed group. The maximal activities of CPT-I, CPT-II, and citrate synthase were all increased in the HI-IT group compared to the Sed group (P < 0.01), as also was gene expression, measured by RT-PCR, of fatty acid binding protein (FABP; P < 0.01) and lipoprotein lipase (LPL; P < 0.05). Lactate dehydrogenase also presented a higher maximal activity (nmol·min -1 ·mg protein ) in HI-IT (around 83%). We suggest that 8 weeks of HI-IT enhance mitochondrial lipid transport capacity thus facilitating the oxidation process in the gastrocnemius muscle. This adaptation may also be associated with the decrease in weight gain observed in the animals and was concomitant to a higher gene expression of both FABP and LPL in HI-IT, suggesting that intermittent exercise is a “time-efficient” strategy inducing metabolic adaptation. Key words: High-intensity intermittent training; Lipid metabolism; Carnitine palmitoyl transferase |
| وصف الملف: | text/html |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::fb8e31132c1b78b953384723048bec84Test http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000800013&lng=en&tlng=enTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....fb8e31132c1b78b953384723048bec84 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |