Putative role of ischemic postconditioning in a rat model of limb ischemia and reperfusion: involvement of hypoxia-inducible factor-1α expression
| العنوان: | Putative role of ischemic postconditioning in a rat model of limb ischemia and reperfusion: involvement of hypoxia-inducible factor-1α expression |
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| المؤلفون: | X.N. Chen, A.X. Zhu, Yong Zhou, Ting Wang |
| المصدر: | Brazilian Journal of Medical and Biological Research v.47 n.9 2014 Brazilian Journal of Medical and Biological Research Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC Brazilian Journal of Medical and Biological Research, Volume: 47, Issue: 9, Pages: 738-745, Published: 25 JUL 2014 Brazilian Journal of Medical and Biological Research, Vol 47, Iss 9, Pp 738-745 (2014) |
| بيانات النشر: | Associação Brasileira de Divulgação Científica, 2014. |
| سنة النشر: | 2014 |
| مصطلحات موضوعية: | Male, Pathology, Time Factors, Physiology, Hindlimb, Biochemistry, Random Allocation, chemistry.chemical_compound, General Pharmacology, Toxicology and Pharmaceutics, Creatine Kinase, lcsh:QH301-705.5, lcsh:R5-920, biology, General Neuroscience, Interleukin, General Medicine, Immunohistochemistry, Interleukin-10, Up-Regulation, medicine.anatomical_structure, Reperfusion Injury, lcsh:Medicine (General), Perfusion, Skeletal muscle ischemia, medicine.medical_specialty, Blotting, Western, Immunology, Biophysics, Ischemia, Ocean Engineering, Internal medicine, Lactate dehydrogenase, medicine, Animals, Rats, Wistar, Muscle, Skeletal, Ischemic postconditioning, L-Lactate Dehydrogenase, Interleukin-6, Tumor Necrosis Factor-alpha, business.industry, Hypoxia-inducible factor-1α, Biomedical Sciences, Endothelial Cells, Skeletal muscle, Extremities, Cell Biology, Hypoxia-Inducible Factor 1, alpha Subunit, medicine.disease, Disease Models, Animal, Endocrinology, lcsh:Biology (General), chemistry, Hypoxia-inducible factor-1?, biology.protein, Creatine kinase, business, Reperfusion injury |
| الوصف: | Hypoxia-inducible factor-1α (HIF-1α) is one of the most potent angiogenic growth factors. It improves angiogenesis and tissue perfusion in ischemic skeletal muscle. In the present study, we tested the hypothesis that ischemic postconditioning is effective for salvaging ischemic skeletal muscle resulting from limb ischemia-reperfusion injury, and that the mechanism involves expression of HIF-1α. Wistar rats were randomly divided into three groups (n=36 each): sham-operated (group S), hindlimb ischemia-reperfusion (group IR), and ischemic postconditioning (group IPO). Each group was divided into subgroups (n=6) according to reperfusion time: immediate (0 h, T0), 1 h (T1), 3 h (T3), 6 h (T6), 12 h (T12), and 24 h (T24). In the IPO group, three cycles of 30-s reperfusion and 30-s femoral aortic reocclusion were carried out before reperfusion. At all reperfusion times (T0-T24), serum creatine kinase (CK) and lactate dehydrogenase (LDH) activities, as well as interleukin (IL)-6, IL-10, and tumor necrosis factor-α (TNF-α) concentrations, were measured in rats after they were killed. Histological and immunohistochemical methods were used to assess the skeletal muscle damage and HIF-1α expression in skeletal muscle ischemia. In groups IR and IPO, serum LDH and CK activities and TNF-α, IL-6, and IL-10 concentrations were all significantly increased compared to group S, and HIF-1α expression was up-regulated (P |
| وصف الملف: | text/html |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1817f9b86c112a7b07fcfb673ab41065Test http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000900738Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....1817f9b86c112a7b07fcfb673ab41065 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |