Crotoxin, the major toxin from the rattlesnake Crotalus durissus terrificus, inhibits ³H-choline uptake in guinea pig ileum
| العنوان: | Crotoxin, the major toxin from the rattlesnake Crotalus durissus terrificus, inhibits ³H-choline uptake in guinea pig ileum |
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| المؤلفون: | L. S. Kattah, Marcelo M. Santoro, M.E. de Lima, Carlos R. Diniz |
| المصدر: | Brazilian Journal of Medical and Biological Research, Vol 33, Iss 9, Pp 1093-1097 (2000) Brazilian Journal of Medical and Biological Research, Volume: 33, Issue: 9, Pages: 1093-1097, Published: SEP 2000 |
| بيانات النشر: | Associação Brasileira de Divulgação Científica, 2000. |
| سنة النشر: | 2000 |
| مصطلحات موضوعية: | guinea pig ileum, Physiology, Immunology, Biophysics, Venom, Phospholipase, Pharmacology, Biology, Neurotransmission, medicine.disease_cause, Biochemistry, chemistry.chemical_compound, Phospholipase A2, Lactate dehydrogenase, medicine, General Pharmacology, Toxicology and Pharmaceutics, Incubation, lcsh:QH301-705.5, Myenteric plexus, lcsh:R5-920, Toxin, General Neuroscience, crotoxin, Cell Biology, General Medicine, choline uptake, Crotalus durissus terrificus, chemistry, lcsh:Biology (General), biology.protein, phospholipase A2, lcsh:Medicine (General) |
| الوصف: | We examined the effect of crotoxin, the neurotoxic complex from the venom of the South American rattlesnake Crotalus durissus terrificus, on the uptake of 3H-choline in minces of smooth muscle myenteric plexus from guinea pig ileum. In the concentration range used (0. 03-1 microM) and up to 10 min of treatment, crotoxin decreased 3H-choline uptake by 50-75% compared to control. This inhibition was time dependent and did not seem to be associated with the disruption of the neuronal membrane, because at least for the first 20 min of tissue exposure to the toxin (up to 1 microM) the levels of lactate dehydrogenase (LDH) released into the supernatant were similar to those of controls. Higher concentrations of crotoxin or more extensive incubation times with this toxin resulted in elevation of LDH activity detected in the assay supernatant. The inhibitory effect of crotoxin on 3H-choline uptake seems to be associated with its phospholipase activity since the equimolar substitution of Sr2+ for Ca2+ in the incubation medium or the modification of the toxin with p-bromophenacyl bromide substantially decreased this effect. Our results show that crotoxin inhibits 3H-choline uptake with high affinity (EC25 = 10 +/- 5 nM). We suggest that this inhibition could explain, at least in part, the blocking effect of crotoxin on neurotransmission. |
| وصف الملف: | text/html |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3b05439dfc80a8f68e733e8389b736feTest http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000900017Test |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....3b05439dfc80a8f68e733e8389b736fe |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |