POD-1/Tcf21 overexpression reduces endogenous SF-1 and StAR expression in rat adrenal cells
| العنوان: | POD-1/Tcf21 overexpression reduces endogenous SF-1 and StAR expression in rat adrenal cells |
|---|---|
| المؤلفون: | Nayara Pereirae Abreu, Claudimara Ferini Pacicco Lotfi, Monica M. França, Talita Aparecida de Moraes Vrechi |
| المصدر: | Brazilian Journal of Medical and Biological Research, Volume: 48, Issue: 12, Pages: 1087-1094, Published: 29 SEP 2015 Brazilian Journal of Medical and Biological Research, Vol 48, Iss 12, Pp 1087-1094 (2015) Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP Brazilian Journal of Medical and Biological Research Brazilian Journal of Medical and Biological Research v.48 n.12 2015 Associação Brasileira de Divulgação Científica (ABDC) instacron:ABDC |
| بيانات النشر: | Associação Brasileira de Divulgação Científica, 2015. |
| سنة النشر: | 2015 |
| مصطلحات موضوعية: | Male, Medicine (General), Physiology, Gene Expression, medicine.disease_cause, Steroidogenic Factor 1, Biochemistry, Rats, Sprague-Dawley, Basic Helix-Loop-Helix Transcription Factors, POD-1, General Pharmacology, Toxicology and Pharmaceutics, Biology (General), lcsh:QH301-705.5, lcsh:R5-920, Adrenal cortex, Reverse Transcriptase Polymerase Chain Reaction, General Neuroscience, Fasciculata cells, General Medicine, Transfection, PROLIFERAÇÃO CELULAR, medicine.anatomical_structure, SF-1, Electrophoresis, Polyacrylamide Gel, Zona Glomerulosa, G cell, lcsh:Medicine (General), Glomerulosa cells, Zona reticularis, QH301-705.5, Immunology, Immunoblotting, Primary Cell Culture, Biophysics, Ocean Engineering, Biology, Real-Time Polymerase Chain Reaction, R5-920, Zona fasciculata, medicine, Animals, RNA, Messenger, Biomedical Sciences, Cell Biology, Phosphoproteins, Molecular biology, Zona Reticularis, Tcf21, lcsh:Biology (General), Cell culture, Zona glomerulosa, Rat adrenal cell primary culture, Adrenal Cortex, Zona Fasciculata, Carcinogenesis |
| الوصف: | During gonad and adrenal development, the POD-1/capsulin/TCF21transcription factor negatively regulates SF-1/NR5A1expression, with higher SF-1 levels being associated with increased adrenal cell proliferation and tumorigenesis. In adrenocortical tumor cells, POD-1 binds to the SF-1 E-box promoter region, decreasing SF-1 expression. However, the modulation of SF-1 expression by POD-1 has not previously been described in normal adrenal cells. Here, we analyzed the basal expression of Pod-1 and Sf-1 in primary cultures of glomerulosa (G) and fasciculata/reticularis (F/R) cells isolated from male Sprague-Dawley rats, and investigated whether POD-1 overexpression modulates the expression of endogenous Sf-1 and its target genes in these cells. POD-1 overexpression, following the transfection of pCMVMycPod-1, significantly decreased the endogenous levels of Sf-1 mRNA and protein in F/R cells, but not in G cells, and also decreased the expression of the SF-1 target StAR in F/R cells. In G cells overexpressing POD-1, no modulation of the expression of SF-1 targets, StAR and CYP11B2, was observed. Our data showing that G and F/R cells respond differently to ectopic POD-1 expression emphasize the functional differences between the outer and inner zones of the adrenal cortex, and support the hypothesis that SF-1 is regulated by POD-1/Tcf21 in normal adrenocortical cells lacking the alterations in cellular physiology found in tumor cells. |
| وصف الملف: | text/html |
| اللغة: | English |
| الوصول الحر: | https://explore.openaire.eu/search/publication?articleId=doi_dedup___::050a54422ba5639c3c753b1219510a50Test http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001201087&lng=en&tlng=enTest |
| حقوق: | OPEN |
| رقم الانضمام: | edsair.doi.dedup.....050a54422ba5639c3c753b1219510a50 |
| قاعدة البيانات: | OpenAIRE |
| الوصف غير متاح. |