دورية أكاديمية
Gene-Based Diagnosis of Tuberculosis from Oral Swabs with a New Generation Pathogen Enrichment Technique
| العنوان: | Gene-Based Diagnosis of Tuberculosis from Oral Swabs with a New Generation Pathogen Enrichment Technique |
|---|---|
| المساهمون: | Young Ae Kang, Bonhan Koo, Ock-Hwa Kim, Joung Ha Park, Ho Cheol Kim, Hyo Joo Lee, Myoung Gyu Kim, Youngwon Jang, Na Hyun Kim, Yong Seo Koo, Yong Shin, Sei Won Lee, Sung-Han Kim, Kang, Young Ae |
| بيانات النشر: | ASM Press |
| سنة النشر: | 2022 |
| مصطلحات موضوعية: | Humans, Middle Aged, Mycobacterium tuberculosis* / genetics, Rifampin / therapeutic use, Sensitivity and Specificity, Tuberculosis* / diagnosis, Tuberculosis* / drug therapy, Tuberculosis, Pulmonary* / microbiology, gene-based diagnosis, oral swab, rapid diagnosis |
| الوصف: | A rapid and sensitive diagnosis is crucial for the management of tuberculosis (TB). A simple and label-free approach via homobifunctional imidoesters with a microfluidic platform (SLIM) assay showed a higher sensitivity than the Xpert MTB/RIF assay in the diagnosis of pulmonary TB (PTB). Here, we evaluated the efficacy of the SLIM assay for oral swab samples from cases of suspected PTB. Patients with clinically suspected PTB were prospectively enrolled and oral swab samples were processed using the SLIM assay and the attending physicians were blinded to the results of the SLIM assay. TB cases were defined as those treated with anti-TB chemotherapy for at least 6 months at the discretion of the specialists based on their clinical features and conventional laboratory results, including the Xpert assay. A total of 272 patients (with TB, n = 128 [47.1%]; without TB, n = 144 [52.9%]; mean age, 59.8 years) were enrolled. Overall, the sensitivity of the oral swab-based SLIM assay (65.6%) was higher than that of the sputum-based Xpert assay (43.4%; P = 0.001). Specifically, the SLIM oral swab assay showed a notably higher sensitivity in culture-negative TB cases compared with the Xpert assay (69.0% [95% CI: 49.2 to 84.7%] versus 7.4% [95% CI: 0.9 to 24.3%]; P = 0.001). The specificity of the SLIM and the Xpert assays was 86.1% (95% CI: 79.3 to 91.3%) and 100% (95% CI: 97.2 to 100%), respectively. When only culture-confirmed cases were analyzed, the SLIM oral swab was comparable to sputum Xpert in sensitivity (64.7% versus 54.3%, P = 0.26). The oral swab-based SLIM assay showed a superior sensitivity for TB diagnosis over the sputum-based Xpert assay, especially for culture-negative cases. IMPORTANCE The development of a rapid, accessible, and highly sensitive diagnostic tool is a major challenge in the control and management of tuberculosis. Gene-based diagnostics is recommended for the rapid diagnosis of pulmonary tuberculosis (PTB), but its sensitivity, such as Xpert MTB/RIF assay (Xpert), drops in cases with a low ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 2165-0497 |
| العلاقة: | MICROBIOLOGY SPECTRUM; J04082; OAK-2022-02786; https://ir.ymlib.yonsei.ac.kr/handle/22282913/189392Test; T202202370; MICROBIOLOGY SPECTRUM, Vol.10(3) : e0020722, 2022-06 |
| DOI: | 10.1128/spectrum.00207-22 |
| الإتاحة: | https://doi.org/10.1128/spectrum.00207-22Test https://ir.ymlib.yonsei.ac.kr/handle/22282913/189392Test |
| حقوق: | CC BY-NC-ND 2.0 KR |
| رقم الانضمام: | edsbas.88674D5F |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 21650497 |
|---|---|
| DOI: | 10.1128/spectrum.00207-22 |