دورية أكاديمية
Microbial plankton community structure and function responses to vitamin B12 and B1 amendments in an upwelling system
| العنوان: | Microbial plankton community structure and function responses to vitamin B12 and B1 amendments in an upwelling system |
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| المؤلفون: | Joglar Quesada, Vanessa, Pontiller, Benjamin, Martínez García, Sandra, Fuentes Lema, Antonio, Pérez Lorenzo, María, Lundin, Daniel, Pinhassi, Jarone, Fernández Suárez, Emilio Manuel, Teira Gonzalez, Eva Maria |
| بيانات النشر: | Applied and Environmental Microbiology Ecoloxía e bioloxía animal Xeociencias mariñas e ordenación do territorio Oceanografía Biolóxica |
| سنة النشر: | 2021 |
| المجموعة: | University of Vigo: Investigo (Repositorio Institucional de la Universidade de Vigo) |
| مصطلحات موضوعية: | 2510.01 Oceanografía Biológica |
| الوصف: | B vitamins are essential cofactors for practically all living organisms on Earth and are produced by a selection of microorganisms. An imbalance between high demand and limited production, in concert with abiotic processes, may explain the low availability of these vitamins in marine systems. Natural microbial communities from surface shelf water in the productive area off northwestern Spain were enclosed in mesocosms in winter, spring, and summer 2016. In order to explore the impact of B-vitamin availability on microbial community composition (16S and 18S rRNA gene sequence analysis) and bacterial function (metatranscriptomics analysis) in different seasons, enrichment experiments were conducted with seawater from the mesocosms. Our findings revealed that significant increases in phytoplankton or prokaryote biomass associated with vitamin B12 and/or B1 amendments were not accompanied by significant changes in community composition, suggesting that most of the microbial taxa benefited from the external B-vitamin supply. Metatranscriptome analysis suggested that many bacteria were potential consumers of vitamins B12 and B1, although the relative abundance of reads related to synthesis was ca. 3.6-fold higher than that related to uptake. Alteromonadales and Oceanospirillales accounted for important portions of vitamin B1 and B12 synthesis gene transcription, despite accounting for only minor portions of the bacterial community. Flavobacteriales appeared to be involved mostly in vitamin B12 and B1 uptake, and Pelagibacterales expressed genes involved in vitamin B1 uptake. Interestingly, the relative expression of vitamin B12 and B1 synthesis genes among bacteria strongly increased upon inorganic nutrient amendment. Collectively, these findings suggest that upwelling events intermittently occurring during spring and summer in productive ecosystems may ensure an adequate production of these cofactors to sustain high levels of phytoplankton growth and biomass. ; Ministerio de Economía y Competitividad | Ref. ... |
| نوع الوثيقة: | article in journal/newspaper |
| اللغة: | English |
| تدمد: | 00992240 10985336 |
| العلاقة: | info:eu-repo/grantAgreement/MINECO//CTM2014-59031-P/ES; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/CTM2017-83362-R/ES; info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-110011RB-C33/ES; Applied and Environmental Microbiology, 87(22): 1 (2021); http://hdl.handle.net/11093/6440Test |
| DOI: | 10.1128/AEM.01525-21 |
| الإتاحة: | https://doi.org/10.1128/AEM.01525-21Test http://hdl.handle.net/11093/6440Test |
| حقوق: | Attribution 4.0 International ; https://creativecommons.org/licenses/by/4.0Test/ ; openAccess |
| رقم الانضمام: | edsbas.1A5FCFE2 |
| قاعدة البيانات: | BASE |
Full Text Finder | تدمد: | 00992240 10985336 |
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| DOI: | 10.1128/AEM.01525-21 |